Logo image
The calcium signal and neutrophil activation
Journal article   Peer reviewed

The calcium signal and neutrophil activation

Karl-Heinz Krause, Kevin P Campbell, Michael J Welsh and Daniel P Lew
Clinical biochemistry, Vol.23(2), pp.159-166
1990
DOI: 10.1016/0009-9120(90)80030-M
PMID: 2197028

View Online

Abstract

The cytosolic free calcium concentration, [Ca 2+] i in phagocytic cells ( e.g. neutrophils, human leukemic cell line HL-60) is an important determinant of cellular activity. In resting phagocytes [Ca 2+] i is low (approximately 100 nM), but in response to occupation of cell surface receptors, it rises to micromolar levels, thereby activating a variety of cellular functions. The increases in [Ca 2+] i consist of two components: an immediate that is independent of extracellular Ca 2+, and a more delayed that is abolished by the removal of extracellular Ca 2+. These two components reflect the involvement of two subcellular structures in intracellular Ca 2+ homeostasis: an intracellular Ca 2+ store, referred to as the calciosome; and the plasma membrane. The function of the intracellular Ca 2+ - store depends on a Ca 2+ -pump, functionally and immunologically related to the cardiac sarcoplasmic reticulum Ca 2+ -ATPase, a Ca 2+ -storage protein, similar to muscle calsequestrin, and a Ca 2+ - release channel, which is sensitive to inositol 1,4,5-trisphosphate. The Ca 2+ -regulatory function of the plasma membrane depends on a Ca 2+ pump, similar to the erythrocyte-type Ca 2+ -ATPase, and a Ca 2+ channel; the activity of the Ca 2+ channel is closely coupled to phosphatidylinositol turnover.
calsequestrin phosphatidylinositol calcium channels calcium homeostasis calciosomes neutrophil granulocytes Ca 2+ - ATPase

Details

Metrics

Logo image