Journal article
The cellular architecture and molecular determinants of the zebrafish fusogenic synapse
Developmental cell, Vol.57(13), pp.1582-1597.e6
07/11/2022
DOI: 10.1016/j.devcel.2022.05.016
PMCID: PMC10180866
PMID: 35709765
Abstract
Myoblast fusion is an indispensable process in skeletal muscle development and regeneration. Studies in Drosophila led to the discovery of the asymmetric fusogenic synapse, in which one cell invades its fusion partner with actin-propelled membrane protrusions to promote fusion. However, the timing and sites of vertebrate myoblast fusion remain elusive. Here, we show that fusion between zebrafish fast muscle cells is mediated by an F-actin-enriched invasive structure. Two cell adhesion molecules, Jam2a and Jam3b, are associated with the actin structure, with Jam2a being the major organizer. The Arp2/3 actin nucle-ation-promoting factors, WAVE and WASP???but not the bipartite fusogenic proteins, Myomaker or Myo-mixer???promote the formation of the invasive structure. Moreover, the convergence of fusogen-containing microdomains and the invasive protrusions is a prerequisite for cell membrane fusion. Thus, our study pro-vides unprecedented insights into the cellular architecture and molecular determinants of the asymmetric fu-sogenic synapse in an intact vertebrate animal.
Details
- Title: Subtitle
- The cellular architecture and molecular determinants of the zebrafish fusogenic synapse
- Creators
- Zhou Luo - The University of Texas Southwestern Medical CenterJun Shi - The University of Texas Southwestern Medical CenterPratima Pandey - The University of Texas Southwestern Medical CenterZhi-Rong Ruan - The University of Texas Southwestern Medical CenterMaria Sevdali - Johns Hopkins UniversityYe Bu - Johns Hopkins UniversityYue Lu - The University of Texas Southwestern Medical CenterShaojun Du - University of Maryland, BaltimoreElizabeth H. Chen - The University of Texas Southwestern Medical Center
- Resource Type
- Journal article
- Publication Details
- Developmental cell, Vol.57(13), pp.1582-1597.e6
- DOI
- 10.1016/j.devcel.2022.05.016
- PMID
- 35709765
- PMCID
- PMC10180866
- NLM abbreviation
- Dev Cell
- ISSN
- 1534-5807
- eISSN
- 1878-1551
- Publisher
- Elsevier
- Number of pages
- 23
- Grant note
- R01AR075005; R01AR053173; R35GM 136316; 1S10OD021685-01A1 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AR053173 / National Institute of Arthritis and Musculoskeletal and Skin Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
- Language
- English
- Date published
- 07/11/2022
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9985150205802771
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