Journal article
The cellular transcription factor USF cooperates with varicella-zoster virus immediate-early protein 62 to symmetrically activate a bidirectional viral promoter
Molecular and cellular biology, Vol.14(10), pp.6896-6906
10/1994
DOI: 10.1128/mcb.14.10.6896-6906.1994
PMCID: PMC359220
PMID: 7935407
Abstract
The mechanisms governing the function of cellular USF and herpesvirus immediate-early transcription factors are subjects of considerable interest. In this regard, we identified a novel form of coordinate gene regulation involving a cooperative interplay between cellular USF and the varicella-zoster virus immediate-early protein 62 (IE 62). A single USF-binding site defines the potential level of IE 62-dependent activation of a bidirectional viral early promoter of the DNA polymerase and major DNA-binding protein genes. We also report a dominant negative USF-2 mutant lacking the DNA-binding domain that permits the delineation of the biological role of both USF-1 and USF-2 in this activation process. The symmetrical stimulation of the bidirectional viral promoter by IE 62 is achieved at concentrations of USF-1 (43 kDa) or USF-2 (44 kDa) already existing in cells. Our observations support the notion that cellular USF can intervene in and possibly target promoters for activation by a herpesvirus immediate-early protein.
Details
- Title: Subtitle
- The cellular transcription factor USF cooperates with varicella-zoster virus immediate-early protein 62 to symmetrically activate a bidirectional viral promoter
- Creators
- Jeffery L Meier - Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892Xu Luo - The University of Texas MD Anderson Cancer CenterMichèle Sawadogo - Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892Stephen E Straus - Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.14(10), pp.6896-6906
- DOI
- 10.1128/mcb.14.10.6896-6906.1994
- PMID
- 7935407
- PMCID
- PMC359220
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 10/1994
- Academic Unit
- Infectious Diseases; Epidemiology; Internal Medicine
- Record Identifier
- 9984094489702771
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