The change in nasal inflammatory markers after intranasal challenges with particulate chitin and lipopolysaccharide: a randomized, double-blind, placebo-controlled, crossover study with a positive control

Torben Sigsgaard; Peter S Thorne; Vivi Schlünssen; Jakob Bønløkke; Ingunn S Riddervold; Kimberly A Hoppe; Niels Trolle Andersen; Neill M Mackenzie

doi:10.1002/alr.21534

Journal article

The change in nasal inflammatory markers after intranasal challenges with particulate chitin and lipopolysaccharide: a randomized, double-blind, placebo-controlled, crossover study with a positive control

Torben Sigsgaard, Peter S Thorne, Vivi Schlünssen, Jakob Bønløkke, Ingunn S Riddervold, Kimberly A Hoppe, Niels Trolle Andersen and Neill M Mackenzie

International forum of allergy & rhinology, Vol.5(8), pp.716-723

08/2015

DOI: 10.1002/alr.21534

PMID: 25851155

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Abstract

We investigated the effect of chitin on the inflammation and immune modulation of the nasal mucosa. This compound was compared to placebo and as a positive control we used lipopolysaccharide (LPS). Fourteen healthy nonsmoking volunteers 22 to 28 years of age were included. All persons underwent exposure to chitin microparticles (CP) and placebo in a randomized double-blinded fashion. In a last session we used LPS from Enterobacter agglomerans in a single-blinded fashion. There were 2 weeks between each session. The outcome measures were Total Nasal Symptom Score (TNSS) and nasal lavage for cytokines and cells at 0, 3, 4, 8 hours. We showed that CP was only weakly inflammatory compared to LPS. In contrast to the LPS response, we did however show an immune-regulatory effect of CP on enhanced interleukin (IL)-4 and IL-6 responses known to downregulate T helper 2 (Th2) responses, indicating a potential beneficial effect of CP for the regulation of the allergic Th2 immune response. This study also shows that CP is well tolerated in healthy volunteers, and that does not induce significantly more symptoms compared to placebo. In fact there is a tendency for CP instillation to induce less rhinorrhoea compared to placebo.

Single-Blind Method

Cell Count

Humans

Lipopolysaccharides - administration & dosage

Male

Th2 Cells - immunology

Healthy Volunteers

Nasal Obstruction - chemically induced

Young Adult

Immunity, Humoral - physiology

Adult

Female

Nasal Mucosa - metabolism

Interleukin-6 - metabolism

Pruritus - chemically induced

Biomarkers - metabolism

Rhinitis - immunology

Cytokines - metabolism

Double-Blind Method

Down-Regulation

Interleukin-4 - metabolism

Administration, Intranasal

Chitin - pharmacology

Cross-Over Studies

Rhinitis - metabolism

Nasal Mucosa - immunology

Lipopolysaccharides - pharmacology

Sneezing - drug effects

Chitin - administration & dosage

Details

Title: Subtitle: The change in nasal inflammatory markers after intranasal challenges with particulate chitin and lipopolysaccharide: a randomized, double-blind, placebo-controlled, crossover study with a positive control
Creators: Torben Sigsgaard - Section of Environment, Occupation and Health, Department of Public Health, University of Aarhus, Aarhus, Denmark
Peter S Thorne - College of Public Health, Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA
Vivi Schlünssen - Section of Environment, Occupation and Health, Department of Public Health, University of Aarhus, Aarhus, Denmark
Jakob Bønløkke - Section of Environment, Occupation and Health, Department of Public Health, University of Aarhus, Aarhus, Denmark
Ingunn S Riddervold - Section of Environment, Occupation and Health, Department of Public Health, University of Aarhus, Aarhus, Denmark
Kimberly A Hoppe - College of Public Health, Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA
Niels Trolle Andersen - Section of Biostatistics, Department of Public Health, University of Aarhus, Aarhus, Denmark
Neill M Mackenzie - Roji Ltd, Welwyn Garden City, Hertfordshire, UK
Resource Type: Journal article
Publication Details: International forum of allergy & rhinology, Vol.5(8), pp.716-723
Publisher: United States
DOI: 10.1002/alr.21534
PMID: 25851155
ISSN: 2042-6976
eISSN: 2042-6984
Grant note: P30 ES005605 / NIEHS NIH HHS
Language: English
Date published: 08/2015
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health
Record Identifier: 9983997448602771

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