Journal article
The characteristics of human cranial bone marrow mesenchymal stem cells
Neuroscience letters, Vol.606, pp.161-166
10/08/2015
DOI: 10.1016/j.neulet.2015.08.056
PMID: 26343934
Abstract
•We established mesenchymal stem cells from human cranial bone marrow.•We confirmed neural crest-associated gene expression of our stem cells.•These cells should have a greater tendency to differentiate into neuronal cells.
Recently, cell-based therapy has attracted attention for treatment of central nervous system (CNS) disorders. Bone marrow-derived mesenchymal stem cells (BMSCs) are considered to have good engraftment potential. Therefore, more efficient and less invasive methods to obtain donor cells are required. Here, we established human BMSCs from cranial bone waste (cBMSCs) obtained following routine neurosurgical procedures.
cBMSCs and cells obtained from the iliac crest (iBMSCs, standard BMSCs) showed expression of cell surface markers associated with mesenchymal stem cells and multipotency traits such as differentiation into osteogenic and adipogenic lineages. cBMSCs showed higher expression of the neural crest-associated mRNAs p75, Slug, and Snail than iBMSCs. Neurogenic induced cells from cBMSCs expressed the neural markers nestin, Pax6, neurofilament (NF)-L, and NF-M as seen with RT-PCR, and NF-M protein as seen with western blotting at higher levels than cells from iBMSCs. Immunostaining showed a significantly greater proportion of NF-M-positive cells in the population of induced cBMSCs compared with the population of iBMSCs. Thus, cBMSCs showed a greater tendency to differentiate into neuron-like cells than iBMSCs.
Details
- Title: Subtitle
- The characteristics of human cranial bone marrow mesenchymal stem cells
- Creators
- Katsuhiro Shinagawa - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanTakafumi Mitsuhara - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanTakahito Okazaki - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanMasaaki Takeda - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanSatoshi Yamaguchi - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanTakuro Magaki - Department of Neurosurgery, Hiroshima Prefectural Hospital, 1-5-54 Ujinakanda, Minami-ku, Hiroshima City, Hiroshima 734-8530, JapanYunosuke Okura - Bio-Environmental Adaptation Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanHiroyuki Uwatoko - Bio-Environmental Adaptation Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, JapanYumi Kawahara - Space Bio-Laboratories Co., Ltd., 1-9-14-503 Danbara-minami, Minami-ku, Hiroshima City, Hiroshima 732-0814, JapanLouis Yuge - Space Bio-Laboratories Co., Ltd., 1-9-14-503 Danbara-minami, Minami-ku, Hiroshima City, Hiroshima 732-0814, JapanKaoru Kurisu - Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8551, Japan
- Resource Type
- Journal article
- Publication Details
- Neuroscience letters, Vol.606, pp.161-166
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/j.neulet.2015.08.056
- PMID
- 26343934
- ISSN
- 0304-3940
- eISSN
- 1872-7972
- Language
- English
- Date published
- 10/08/2015
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984040260702771
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