The chlorate-resistant and photomorphogenesis-defective mutant cr88 encodes a chloroplast-targeted HSP90

Dongsun Cao; John E Froehlich; Hui Zhang; Chi-Lien Cheng

doi:10.1046/j.1365-313X.2003.016011.x

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The chlorate-resistant and photomorphogenesis-defective mutant cr88 encodes a chloroplast-targeted HSP90

Journal article

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The chlorate-resistant and photomorphogenesis-defective mutant cr88 encodes a chloroplast-targeted HSP90

Dongsun Cao, John E Froehlich, Hui Zhang and Chi-Lien Cheng

The Plant journal : for cell and molecular biology, Vol.33(1), pp.107-118

01/2003

DOI: 10.1046/j.1365-313X.2003.016011.x

PMID: 12943545

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Abstract

The cr88 mutant of Arabidopsis is a novel chlorate-resistant mutant that displays long hypocotyls in red light, but not in far red or blue light, and is delayed in the greening process. In cotyledons and young leaves, plastids are less developed compared with those of the wild type. In addition, a subset of light-regulated genes are under-expressed in this mutant. To understand the pleiotropic phenotypes of cr88, we isolated the CR88 gene through map-based cloning. We found that CR88 encodes a chloroplast-targeted 90-kDa heat shock protein (HSP90). The CR88 gene is expressed at highest levels during early post-germination stages and in leaves and reproductive organs. It is constitutively expressed but is also light and heat shock inducible. Chloroplast import experiments showed that the protein is localized to the stroma compartment of the chloroplast. The possible function of an HSP90 in the chloroplast and a plausible explanation of the pleiotropic phenotypes observed in cr88 are discussed.

Morphogenesis

Mutagenesis

Phenotype

Amino Acid Sequence

Arabidopsis - genetics

Arabidopsis - growth & development

Arabidopsis Proteins - chemistry

Arabidopsis Proteins - genetics

Base Sequence

Chloroplasts - genetics

HSP90 Heat-Shock Proteins - chemistry

HSP90 Heat-Shock Proteins - genetics

Light

Molecular Sequence Data

Recombination, Genetic

RNA Probes

Sequence Alignment

Sequence Homology, Amino Acid

Time Factors

Details

Title: Subtitle: The chlorate-resistant and photomorphogenesis-defective mutant cr88 encodes a chloroplast-targeted HSP90
Creators: Dongsun Cao - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
John E Froehlich
Hui Zhang - University of Iowa
Chi-Lien Cheng
Resource Type: Journal article
Publication Details: The Plant journal : for cell and molecular biology, Vol.33(1), pp.107-118
DOI: 10.1046/j.1365-313X.2003.016011.x
PMID: 12943545
NLM abbreviation: Plant J
ISSN: 0960-7412
eISSN: 1365-313X
Language: English
Date published: 01/2003
Academic Unit: Biology
Record Identifier: 9984217534702771

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