Journal article
The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination
Oral microbiology and immunology, Vol.10(5), pp.311-318
1995
DOI: 10.1111/j.1399-302X.1995.tb00160.x
PMID: 8596675
Abstract
It has been suggested that Porphyromonas gingivalis may possess more than one hemagglutinin. We have previously reported the cloning of a gene (hagA) that encodes a hemagglutinin. In this study we report the cloning, characterization, and sequencing of a second gene (hagB) that encodes a protein that also appears to be involved in hemagglutination. Antiserum to the clone (ST 7) was found to inhibit hemagglutination by P. gingivalis 381, and hemagglutinating inhibition activity of anti-P. gingivalis antiserum was reduced by adsorption of the antiserum with cells of clone ST 7. Restriction mapping and Southern analysis indicates there is little or no DNA homology between this cloned 4.8-kb Hind DNA fragment and a cloned hemagglutinin gene we have previously described. Minicell analysis of the cloned P. gingivalis chromosomal DNA fragment revealed that the major gene product is a 49-kDa protein. Immunoaffinity chromotography using purified rabbit immunoglobulin G against the cloned protein resulted in the purification of a major reactive 49- to 50-kDa protein from a P. gingivalis cell lysate. Nucleotide sequence analysis revealed the hagB open reading frame to be 1053 nucleotides in length with a mol% G+C of 59.9% coding for a protein of 350 residues with a calculated molecular weight of 39.375 kDa. This protein was also determined to be basic and hydrophilic and to contain a potential signal peptide. Comparison of both the nucleotide and derived amino acid sequences with computer-based databases did not reveal any significant homologies between hagB and any other previously sequenced genes.
Details
- Title: Subtitle
- The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination
- Creators
- A PROGULSKE-FOX - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesS TUMWASORN - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesG LEPINE - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesJ WHITLOCK - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesD SAVETT - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesJ. J FERRETTI - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United StatesJ. A BANAS - Univ. Florida, coll. dentistry, dep. oral biology, Gainesville FL 32610-0424, United States
- Resource Type
- Journal article
- Publication Details
- Oral microbiology and immunology, Vol.10(5), pp.311-318
- Publisher
- Blackwell; Oxford
- DOI
- 10.1111/j.1399-302X.1995.tb00160.x
- PMID
- 8596675
- ISSN
- 0902-0055
- eISSN
- 1399-302X
- Language
- English
- Date published
- 1995
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984065715302771
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