Journal article
The comet assay in biomonitoring of occupational exposure in rubber factory and plastic lamination plant. Comparison with cytogenetic and immune Biomarkers
Neoplasma, Vol.46(1), pp.23-25
01/01/1999
Abstract
The alkaline comet assay was used in parallel with chromosome aberrations as well as immunological endpoints to assess the effect of exposure in two factories in Slovakia - a rubber factory and a plastic lamination plant. We examined 29 exposed workers from the rubber factory exposed to a complex mixture of contaminants belonging mostly to polycyclic aromatic hydrocarbons, and two control groups (22 subjects each). In the lamination plant, styrene was the only contaminant at the workplace. Monitoring in this plant consisted of 17 hand-laminators directly exposed to styrene, 10 medium exposed sprayers and 18 controls.
In both factories exposed workers had significantly elevated levels of DNA breaks compared with controls (p<0.001). In both studies we found a correlation between strand breaks in lymphocytes and years of exposure (p<0.001, r=0.57, p<0.001, r=0.545 resp.). Cytogenetic results also showed significantly higher numbers of chromosome aberrations in both exposed populations. We observed a clear correlation of chromosome aberrations with DNA strand breaks.
Immune parameters were also changed in workers exposed to both the single contaminant (styrene) and the complex mixture.
Details
- Title: Subtitle
- The comet assay in biomonitoring of occupational exposure in rubber factory and plastic lamination plant. Comparison with cytogenetic and immune Biomarkers
- Creators
- M SomorovskaJ TulinskaM BarancokovaM ZamecnikovaA CollinsA LiskovaB VallovaH PetrovskaE JahnovaP VodickaL FuortesM Dusinska
- Resource Type
- Journal article
- Publication Details
- Neoplasma, Vol.46(1), pp.23-25
- Publisher
- Slovak Academic Press Ltd
- ISSN
- 0028-2685
- Number of pages
- 3
- Language
- English
- Date published
- 01/01/1999
- Academic Unit
- Occupational and Environmental Health; Internal Medicine
- Record Identifier
- 9984364408102771
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