Journal article
The conservation and regulation of rat DSP-PP gene
Connective tissue research, Vol.43(2-3), pp.331-337
2002
DOI: 10.1080/03008200290001041
PMID: 12489177
Abstract
Two highly expressed noncollagenous proteins associated with dentin mineralization, dentin sialoprotein (DSP) and phosphophoryn (PP), are encoded by a single DSP-PP transcript. To better understand how DSP-PP transcripts are regulated, we have determined the DSP-PP transcription start site, sequenced its 5' flanking region, and analyzed the transcriptional activity of the gene promoter out to -1615 bp. Comparison of the rat cDNA sequence with the mouse, rat and human genes clearly indicates high sequence conservation within the DSP-PP 5' flanking region, implicating the possible presence of highly conserved gene regulatory cis elements. Among a number of conserved transcription sites identified in the 5' flanking region, we demonstrate that the conserved Y box sequence (ATTGG) can specifically bind nuclear extracts from mouse MDPC23 cells. This sequence (located within the -57 bp/-52 bp 5' flanking region) therefore likely represents one DSP-PP transcriptional regulatory sequence.
Details
- Title: Subtitle
- The conservation and regulation of rat DSP-PP gene
- Creators
- H H Ritchie - University of Michigan–Ann ArborX R Li - University of Michigan–Ann ArborC T Hanks - University of Michigan–Ann ArborK Knudtson - University of Michigan–Ann ArborL H Wang - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Connective tissue research, Vol.43(2-3), pp.331-337
- DOI
- 10.1080/03008200290001041
- PMID
- 12489177
- ISSN
- 0300-8207
- eISSN
- 1607-8438
- Grant note
- DE 11442 / NIDCR NIH HHS HL-60625 / NHLBI NIH HHS
- Language
- English
- Date published
- 2002
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627324002771
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