Journal article
The contrasting phenotypes of neutrophils during asymptomatic versus symptomatic Leishmania braziliensis infection
The Journal of infectious diseases, Vol.230(6), pp.e1385-e1393
12/16/2024
DOI: 10.1093/infdis/jiae317
PMCID: PMC11646601
PMID: 38912968
Appears in UI Libraries Support Open Access
Abstract
The mechanisms that mediate immune protection in individuals with subclinical (SC) or asymptomatic infection with L. braziliensis are largely unknown. Neutrophils (PMNs) have been implicated in progressive symptomatic cutaneous leishmaniasis (CL), but their potential participation in maintenance of subclinical infection is unexplored. The aim of this study was to compare the phenotypic and functional profiles of PMNs in individuals with SC infection versus patients with symptomatic CL due to L. braziliensis.BACKGROUNDThe mechanisms that mediate immune protection in individuals with subclinical (SC) or asymptomatic infection with L. braziliensis are largely unknown. Neutrophils (PMNs) have been implicated in progressive symptomatic cutaneous leishmaniasis (CL), but their potential participation in maintenance of subclinical infection is unexplored. The aim of this study was to compare the phenotypic and functional profiles of PMNs in individuals with SC infection versus patients with symptomatic CL due to L. braziliensis.Subjects were recruited in the endemic region of Corte de Pedra, Bahia, Brazil. Surface markers to define activation status were characterized by flow cytometry. Functional responses of PMNs including phagocytic capacity, production of oxidative species, and oxidative killing of intracellular parasites were studied in vitro.METHODSSubjects were recruited in the endemic region of Corte de Pedra, Bahia, Brazil. Surface markers to define activation status were characterized by flow cytometry. Functional responses of PMNs including phagocytic capacity, production of oxidative species, and oxidative killing of intracellular parasites were studied in vitro.PMNs from individuals with SC infection displayed a more activated phenotype and greater ability to control the infection than PMNs from patients with CL. In contrast, PMNs from patients with CL exhibited higher expression of HLA-DR and higher production of oxidative species than PMNs from subjects with SC infection.RESULTSPMNs from individuals with SC infection displayed a more activated phenotype and greater ability to control the infection than PMNs from patients with CL. In contrast, PMNs from patients with CL exhibited higher expression of HLA-DR and higher production of oxidative species than PMNs from subjects with SC infection.PMNs from individuals with SC infection can control the infection more efficiently than PMNs from patients with CL, despite the lower production of oxidants. Our observations suggest that L. braziliensis may evade microbicidal mechanisms of PMNs from patients with CL, contributing to parasite dissemination and the establishment of disease.CONCLUSIONPMNs from individuals with SC infection can control the infection more efficiently than PMNs from patients with CL, despite the lower production of oxidants. Our observations suggest that L. braziliensis may evade microbicidal mechanisms of PMNs from patients with CL, contributing to parasite dissemination and the establishment of disease.
Details
- Title: Subtitle
- The contrasting phenotypes of neutrophils during asymptomatic versus symptomatic Leishmania braziliensis infection
- Creators
- Jacilara A Conceiҫão - University of Iowa, Internal MedicinePedro P Carneiro - Complexo Hospitalar Universitário Professor Edgard SantosAndreza S Dórea - Complexo Hospitalar Universitário Professor Edgard SantosWalker N Oliveira - Complexo Hospitalar Universitário Professor Edgard SantosAline C Muniz - Complexo Hospitalar Universitário Professor Edgard SantosEdgar M Carvalho - Fundação Oswaldo CruzMary E Wilson - University of IowaOlívia Bacellar - Complexo Hospitalar Universitário Professor Edgard Santos
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.230(6), pp.e1385-e1393
- DOI
- 10.1093/infdis/jiae317
- PMID
- 38912968
- PMCID
- PMC11646601
- NLM abbreviation
- J Infect Dis
- ISSN
- 1537-6613
- eISSN
- 1537-6613
- Publisher
- Oxford University Press
- Language
- English
- Electronic publication date
- 06/24/2024
- Date published
- 12/16/2024
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984649149002771
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