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The coronavirus nucleocapsid protein is ADP-ribosylated
Journal article   Open access   Peer reviewed

The coronavirus nucleocapsid protein is ADP-ribosylated

Matthew E Grunewald, Anthony R Fehr, Jeremiah Athmer and Stanley Perlman
Virology, Vol.517, pp.62-68
04/2018
DOI: 10.1016/j.virol.2017.11.020
PMCID: PMC5871557
PMID: 29199039
url
https://doi.org/10.1016/j.virol.2017.11.020View
Published (Version of record) Open Access

Abstract

ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated. ADP-ribosylation of N protein was also observed in cells exogenously expressing N protein by transduction using Venezuelan equine encephalitis virus replicon particles (VRPs). However, plasmid-derived N protein was not ADP-ribosylated following transient transfection but was ADP-ribosylated after MHV infection, indicating that this modification requires virus infection. In conclusion, we have identified a novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. •Mouse hepatitis virus nucleocapsid protein is ADP-ribosylated in virions and cells.•ADP-ribosylation of N protein occurs during infection but not after transfection.•ADP-ribosylation of N protein is conserved across many lineages of coronaviruses.
 Macrodomain PEDV Coronavirus Nucleocapsid SARS-CoV MERS-CoV Mouse hepatitis virus ADP-ribosylation

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