The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain

Katherine J D Ashbourne Excoffon; Thomas Moninger; Joseph Zabner

doi:10.1128/JVI.77.4.2559-2567.2003

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The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain

Journal article

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Peer reviewed

The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain

Katherine J D Ashbourne Excoffon, Thomas Moninger and Joseph Zabner

Journal of virology, Vol.77(4), pp.2559-2567

02/2003

DOI: 10.1128/JVI.77.4.2559-2567.2003

PMCID: PMC141093

PMID: 12551994

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Abstract

The coxsackie B virus and adenovirus receptor (CAR) is a member of the immunoglobulin superfamily. In addition to activity as a viral receptor, it may play a role in cellular adhesion. We asked what determines the cell membrane microdomain of CAR. We found that CAR is localized to a novel lipid-rich microdomain similar to that of the low-density lipoprotein receptor (LDLR) but distinct from that of a CAR variant that exhibited traditional lipid raft localization via fusion to a glycosylphosphatidylinositol (GPI) tail. The cytoplasmic tail determines its membrane localization, since deletion of this domain resulted in mislocalization. Results indicate that CAR, CAR-LDLR, and LDLR reside in a novel lipid raft that is distinct from caveolin-1-containing caveolae and GPI-linked proteins. Residence in a lipid-rich domain provides a mechanism that allows CAR to interact with other cell adhesion proteins and yet function as an adenovirus receptor.

Cricetinae

Membrane Microdomains - metabolism

Receptors, Virus - metabolism

Humans

Membrane Microdomains - chemistry

Receptors, LDL - metabolism

Adenoviridae - pathogenicity

Coxsackie and Adenovirus Receptor-Like Membrane Protein

Receptors, Virus - genetics

Microscopy, Electron

Clathrin - pharmacology

Endocytosis

Animals

Receptors, Virus - chemistry

Cell Membrane - metabolism

Adenoviridae - metabolism

COS Cells

Membrane Microdomains - genetics

CHO Cells

Details

Title: Subtitle: The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain
Creators: Katherine J D Ashbourne Excoffon - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Thomas Moninger
Joseph Zabner
Resource Type: Journal article
Publication Details: Journal of virology, Vol.77(4), pp.2559-2567
DOI: 10.1128/JVI.77.4.2559-2567.2003
PMID: 12551994
PMCID: PMC141093
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Grant note: DK 54759 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS
Language: English
Date published: 02/2003
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984094728302771

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