Journal article
The determination of growth rates of individual colonies in agarose using high‐resolution automated image analysisx
Cytometry (New York, N.Y.), Vol.11(7), pp.793-804
1990
DOI: 10.1002/cyto.990110705
Abstract
This paper describes the evaluation of a colony formation assay using automated image analysis, which permits the tracking of growth at the individual colony level, such that a growth rate can be estimated for each colony followed. In principle, this will permit quantitative characterization of cellular heterogeneity in growth rate and cellular heterogeneity in response to proliferation‐modifying agents. In addition, we have demonstrated the possibility of using correlative microscopy to relate growth rate to other parameters, using metabolic viability as an example. This should be useful for determining cellular characteristics associated with proliferative behavior and response to proliferation‐modifying agents.
Details
- Title: Subtitle
- The determination of growth rates of individual colonies in agarose using high‐resolution automated image analysisx
- Creators
- Harry K. Slocum - Roswell Park Cancer InstituteMartin Malmberg - Chaperone TherapeuticsWilliam R. Greco - Roswell Park Cancer InstituteJohn C. Parsons - Roswell Park Cancer InstituteYoucef M. Rustum - Chaperone Therapeutics
- Resource Type
- Journal article
- Publication Details
- Cytometry (New York, N.Y.), Vol.11(7), pp.793-804
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/cyto.990110705
- ISSN
- 0196-4763
- eISSN
- 1097-0320
- Number of pages
- 12
- Language
- English
- Date published
- 1990
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359798902771
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