The effect of acetylglyceryl ether phosphorylcholine on glycogenolysis and phosphatidylinositol 4,5-bisphosphate metabolism in rat hepatocytes

Rory A Fisher; Shivendra D Shukla; Michael S Debuysere; Donald J Hanahan; Merle S Olson

doi:10.1016/S0021-9258(17)47205-X

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The effect of acetylglyceryl ether phosphorylcholine on glycogenolysis and phosphatidylinositol 4,5-bisphosphate metabolism in rat hepatocytes

Journal article

Open access

Peer reviewed

The effect of acetylglyceryl ether phosphorylcholine on glycogenolysis and phosphatidylinositol 4,5-bisphosphate metabolism in rat hepatocytes

Rory A Fisher, Shivendra D Shukla, Michael S Debuysere, Donald J Hanahan and Merle S Olson

The Journal of biological chemistry, Vol.259(14), pp.8685-8688

07/25/1984

DOI: 10.1016/S0021-9258(17)47205-X

PMID: 6086606

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Abstract

The effect of acetylglyceryl ether phosphorylcholine (AGEPC) on glycogenolysis and phosphatidylinositol 4,5-bisphosphate has been studied in rat hepatocytes. Previously, this laboratory demonstrated that AGEPC stimulated glucose output from the perfused rat liver and promoted the breakdown of phosphoinositides in rat hepatocytes (Shukla, S. D., Buxton, D. B., Olson, M.S., and Hanahan, D.J. (1983) J. Biol. Chem. 258, 10212-10214). In the present study, addition of AGEPC (10(-13) to 10(-9) M) to rat hepatocytes failed to stimulate glucose output, whereas epinephrine (10(-5)M) and glucagon (10(-7)M) stimulated glucose output by 100% or more in these same cells. The effects of AGEPC, epinephrine, vasopressin, and glucagon on glycogen phosphorylase activity and the breakdown of phosphatidylinositol 4,5-bisphosphate were compared in hepatocytes. AGEPC (10(-9)M) promoted the breakdown of phosphatidylinositol 4,5-bisphosphate in a fashion similar to epinephrine (10(-5)M) and vasopressin (10(-7)M). In contrast to the two calcium-mobilizing hormones, epinephrine and vasopressin, AGEPC did not cause an activation of glycogen phosphorylase. Glucagon activation of glycogen phosphorylase was not accompanied by a significant effect on phosphatidylinositol 4,5-bisphosphate hydrolysis. Thus, AGEPC is a chemical mediator which induces the degradation of phosphatidylinositol 4,5-bisphosphate without activating glycogenolysis in hepatocytes.

Phosphatidylinositol 4,5-Diphosphate

Phospholipid Ethers

Liver - metabolism

Rats

Vasopressins - pharmacology

Male

Rats, Inbred Strains

Glucagon - pharmacology

Animals

Liver Glycogen - metabolism

Liver - drug effects

Phosphatidylethanolamines - pharmacology

Phosphatidylinositols - metabolism

Kinetics

In Vitro Techniques

Epinephrine - pharmacology

Details

Title: Subtitle: The effect of acetylglyceryl ether phosphorylcholine on glycogenolysis and phosphatidylinositol 4,5-bisphosphate metabolism in rat hepatocytes
Creators: Rory A Fisher
Shivendra D Shukla
Michael S Debuysere
Donald J Hanahan
Merle S Olson
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.259(14), pp.8685-8688
DOI: 10.1016/S0021-9258(17)47205-X
PMID: 6086606
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: AM-33538 / NIADDK NIH HHS
Language: English
Date published: 07/25/1984
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040249502771

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