Journal article
The effect of dimethyl sulfoxide (DMSO) on dentin bonding and nanoleakage of etch-and-rinse adhesives
Dental materials, Vol.29(10), pp.1055-1062
10/2013
DOI: 10.1016/j.dental.2013.07.014
PMID: 23942144
Abstract
The objective was to examine the effect of a solvent dimethyl sulfoxide (DMSO) on resin–dentin bond durability, as well as potential functional mechanisms behind the effect.
Microtensile bond strength (μTBS) was evaluated in extracted human teeth in two separate experiments. Dentin specimens were acid-etched and assigned to pre-treatment with 0.5mM (0.004%) DMSO as additional primer for 30s and to controls with water pre-treatment. Two-step etch-and-rinse adhesive (Scotchbond 1XT, 3M ESPE) was applied and resin composite build-ups were created. Specimens were immediately tested for μTBS or stored in artificial saliva for 6 and 12 months prior to testing. Additional immediate and 6-month specimens were examined for interfacial nanoleakage analysis under SEM. Matrix metalloproteinase (MMP) inhibition by DMSO was examined with gelatin zymography. Demineralized dentin disks were incubated in 100% DMSO to observe the optical clearing effect.
The use of 0.5mM DMSO had no effect on immediate bond strength or nanoleakage. In controls, μTBS decreased significantly after storage, but increased significantly in DMSO-treated group. The control group had significantly lower μTBS than DMSO-group after 6 and 12 months. DMSO also eliminated the increase in nanoleakage seen in controls. 5% and higher DMSO concentrations significantly inhibited the gelatinases. DMSO induced optical clearing effect demonstrating collagen dissociation.
DMSO as a solvent may be useful in improving the preservation of long-term dentin–adhesive bond strength. The effect may relate to dentinal enzyme inhibition or improved wetting of collagen by adhesives. The collagen dissociation required much higher DMSO concentrations than the 0.5mM DMSO used for bonding.
Details
- Title: Subtitle
- The effect of dimethyl sulfoxide (DMSO) on dentin bonding and nanoleakage of etch-and-rinse adhesives
- Creators
- Leo Tjäderhane - Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, FinlandPekka Mehtälä - Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, FinlandPolliana Scaffa - Estate University of Campinas, Piracicaba Dental School (FOP/UNICAMP), São Paulo, BrazilCristina Vidal - Estate University of Campinas, Piracicaba Dental School (FOP/UNICAMP), São Paulo, BrazilVirve Pääkkönen - Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, FinlandLorenzo Breschi - Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, ItalyJosimeri Hebling - Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University – UNESP, Araraquara, SP, BrazilFranklin R Tay - Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA, USAFabio D Nascimento - Bandeirante University of São Paulo (UNIBAN), São Paulo, BrazilDavid H Pashley - Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA, USAMarcela R Carrilho - Bandeirante University of São Paulo (UNIBAN), São Paulo, Brazil
- Resource Type
- Journal article
- Publication Details
- Dental materials, Vol.29(10), pp.1055-1062
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.dental.2013.07.014
- PMID
- 23942144
- ISSN
- 0109-5641
- eISSN
- 1879-0097
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984066098602771
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