Journal article
The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system
Biotechnology and bioengineering, Vol.77(2), pp.219-224
01/20/2002
DOI: 10.1002/bit.10131
PMID: 11753929
Abstract
The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell-baculovirus expression system was investigated in a well-controlled bioreactor. Oligomannose-type N-linked glycans (i.e., Man2 to Man6 and Man3F) were present in SEAP produced by Spodoptera frusiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines. The relative amounts of the most highly processed glycans (i.e., Man3F and Man2 in the SEAP from Sf-9 and Tn-5B1-4 cells, respectively) were significantly higher at 50% of air saturation than at either 10% or 190% of air saturation. That is, glycan processing was inhibited at both low and high dissolved oxygen concentrations.
Details
- Title: Subtitle
- The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system
- Creators
- Fuming Zhang - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USAMark A SaarinenLaura J ItleSybil C LangDavid W MurhammerRobert J Linhardt
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.77(2), pp.219-224
- Publisher
- United States
- DOI
- 10.1002/bit.10131
- PMID
- 11753929
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Language
- English
- Date published
- 01/20/2002
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003424102771
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