Journal article
The effect of elevated glucose levels on myo-inositol metabolism in cultured bovine aortic endothelial cells
Metabolism, clinical and experimental, Vol.38(1), pp.16-22
1989
DOI: 10.1016/0026-0495(89)90174-1
PMID: 2491899
Abstract
Bovine aorta endothelial cells were used to determine the effect of high ambient glucose concentrations on
myo-inositol metabolism. Culturing the cells for a minimum of 1 week in elevated glucose concentrations caused an increase in the intracellular sorbitol content and a decrease in
myo-inositol levels. The accumulation of
myo-inositol from the medium and incorporation into phospholipids was reduced 25% to 50% in cells grown in the presence of 30 to 50 mmol/L glucose. This effect was not observed following a short-term exposure of the cells to elevated glucose levels. Kinetic analysis of high-affinity
myo-inositol uptake showed that the K'm was significantly increased in cells grown in 30 mmol/L glucose compared to those cultured in 5.6 mmol/L glucose. This would suggest that exposing endothelial cells to high ambient glucose levels for a minimum of 1 week leads to a competitive type of inhibition of high-affinity
myo-inositol uptake. The changes in
myo-inositol metabolism and content and sorbitol levels mediated by glucose exposure were blocked by addition of the aldose reductase inhibitor, sorbinil, to the media, suggesting that these changes are caused by the accumulation of sorbitol by the cells. Exposure of bovine aorta endothelial cells to high ambient levels of glucose leads to accumulation of sorbitol in the cells, which is responsible for alterations in
myo-inositol metabolism. These changes could result in alteration of endothelial cell membrane function and contribute to the pathology of diabetes mellitus.
Details
- Title: Subtitle
- The effect of elevated glucose levels on myo-inositol metabolism in cultured bovine aortic endothelial cells
- Creators
- Mark A Yorek - Department of Internal Medicine, Diabetes Endocrinology Research Center, University of Iowa, Iowa City, IA, USAJoyce A Dunlap - Department of Internal Medicine, Diabetes Endocrinology Research Center, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Metabolism, clinical and experimental, Vol.38(1), pp.16-22
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0026-0495(89)90174-1
- PMID
- 2491899
- ISSN
- 0026-0495
- eISSN
- 1532-8600
- Language
- English
- Date published
- 1989
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094706602771
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