Journal article
The effect of smoking on DNA methylation of peripheral blood mononuclear cells from African American women
BMC genomics, Vol.15(1), pp.151-151
02/22/2014
DOI: 10.1186/1471-2164-15-151
PMCID: PMC3936875
PMID: 24559495
Abstract
Background: Regular smoking is associated with a wide variety of syndromes with prominent inflammatory components such as cancer, obesity and type 2 diabetes. Heavy regular smoking is also associated with changes in the DNA methylation of peripheral mononuclear cells. However, in younger smokers, inflammatory epigenetic findings are largely absent which suggests the inflammatory response(s) to smoking may be dose dependent. To help understand whether peripheral mononuclear cells have a role in mediating these responses in older smokers with higher cumulative smoke exposure, we examined genome-wide DNA methylation in a group of well characterized adult African American subjects informative for smoking, as well as serum C-reactive protein (CRP) and interleukin-6 receptor (IL6R) levels. In addition, complementary bioinformatic analyses were conducted to delineate possible pathways affected by long-term smoking.
Results: Genome-wide DNA methylation analysis with respect to smoking status yielded 910 significant loci after Benjamini-Hochberg correction. In particular, two loci from the AHRR gene (cg05575921 and cg23576855) and one locus from the GPR15 gene (cg19859270) were identified as highly significantly differentially methylated between smokers and non-smokers. The bioinformatic analyses showed that long-term chronic smoking is associated with altered promoter DNA methylation of genes coding for proteins mapping to critical sub-networks moderating inflammation, immune function, and coagulation.
Conclusions: We conclude that chronic regular smoking is associated with changes in peripheral mononuclear cell methylation signature which perturb inflammatory and immune function pathways and may contribute to increased vulnerability for complex illnesses with inflammatory components.
Details
- Title: Subtitle
- The effect of smoking on DNA methylation of peripheral blood mononuclear cells from African American women
- Creators
- Meeshanthini V Dogan - Department of Psychiatry, University of Iowa, Rm 2-126 MEB, 500 Newton Road, Iowa City, IA 52242, USABridget Shields - Department of Psychiatry, University of Iowa, Rm 2-126 MEB, 500 Newton Road, Iowa City, IA 52242, USACarolyn Cutrona - Department of Psychology, Iowa State University, Ames, IA, USALong Gao - Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USAFrederick X Gibbons - Department of Psychology, University of Connecticut, Storrs, CT, USARonald Simons - Department of Sociology, University of Georgia, Athens, GA, USAMartha Monick - Department of Internal Medicine, University of Iowa, Iowa City, IA, USAGene H Brody - Center for Family Research, University of Georgia, Athens, GA, USAKai Tan - Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USASteven RH Beach - Center for Family Research, University of Georgia, Athens, GA, USARobert A Philibert - Department of Psychiatry, University of Iowa, Rm 2-126 MEB, 500 Newton Road, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- BMC genomics, Vol.15(1), pp.151-151
- DOI
- 10.1186/1471-2164-15-151
- PMID
- 24559495
- PMCID
- PMC3936875
- NLM abbreviation
- BMC Genomics
- ISSN
- 1471-2164
- eISSN
- 1471-2164
- Publisher
- BioMed Central
- Language
- English
- Date published
- 02/22/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pulmonary, Critical Care, and Occupational Medicine; Psychiatry; Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984070233002771
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