The effect of smoking on MAOA promoter methylation in DNA prepared from lymphoblasts and whole blood

Robert A Philibert; Steven R H Beach; Tracy D Gunter; Gene H Brody; Anup Madan; Meg Gerrard

doi:10.1002/ajmg.b.31031

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The effect of smoking on MAOA promoter methylation in DNA prepared from lymphoblasts and whole blood

Journal article

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Peer reviewed

The effect of smoking on MAOA promoter methylation in DNA prepared from lymphoblasts and whole blood

Robert A Philibert, Steven R H Beach, Tracy D Gunter, Gene H Brody, Anup Madan and Meg Gerrard

American journal of medical genetics. Part B, Neuropsychiatric genetics, Vol.153B(2), pp.619-628

03/05/2010

DOI: 10.1002/ajmg.b.31031

PMCID: PMC3694401

PMID: 19777560

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https://www.ncbi.nlm.nih.gov/pmc/articles/3694401View

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Abstract

Prior work using lymphoblast DNA prepared from 192 subjects from the Iowa Adoption Studies (IAS) demonstrated that decreased MAOA promoter methylation was associated with lifetime symptom count for nicotine dependence (ND) and provided suggestive evidence that the amount of methylation is genotype dependent. In the current investigation, we replicate and extend these prior findings in three ways using another 289 IAS subjects and the same methodologies. First, we show that methylation is dependent on current smoking status. Second, we introduce a factor analytic approach to DNA methylation, highlighting three distinct regions of the promoter that may function in somewhat different ways for males and females. Third, we directly compare the methylation signatures in DNA prepared from whole blood and lymphoblasts from a subset of these subjects and provide suggestive evidence favoring the use of lymphoblast DNA. We conclude that smoking reliably decreases MAOA methylation, but exact characterization of effects on level of methylation depend on genotype, smoking history, current smoking status, gender, and region of the promoter-associated CpG Island examined.

Lymphocytes - metabolism

Promoter Regions, Genetic

Epigenesis, Genetic

Humans

Middle Aged

Genotype

Male

Monoamine Oxidase - genetics

DNA Methylation

Sex Factors

CpG Islands

Adult

Female

Models, Genetic

Smoking

Details

Title: Subtitle: The effect of smoking on MAOA promoter methylation in DNA prepared from lymphoblasts and whole blood
Creators: Robert A Philibert - Neuroscience and Genetics Programs, The University of Iowa, Iowa City, Iowa
Steven R H Beach - Institute for Behavioral Research, The University of Georgia, Athens, Georgia
Tracy D Gunter - Department of Psychiatry, The University of Iowa, Iowa City, Iowa
Gene H Brody - Institute for Behavioral Research, The University of Georgia, Athens, Georgia
Anup Madan - The Swedish Hospital Institute for Neuroscience, Seattle, Washington
Meg Gerrard - Norris Cotton Cancer Center, Dartmouth Medical Center, Lebanon, New Hampshire
Resource Type: Journal article
Publication Details: American journal of medical genetics. Part B, Neuropsychiatric genetics, Vol.153B(2), pp.619-628
DOI: 10.1002/ajmg.b.31031
PMID: 19777560
PMCID: PMC3694401
NLM abbreviation: Am J Med Genet B Neuropsychiatr Genet
ISSN: 1552-4841
eISSN: 1552-485X
Publisher: United States
Grant note: R01 DA019230 / NIDA NIH HHS P30 DA027827 / NIDA NIH HHS DA015789 / NIDA NIH HHS DA02173603 / NIDA NIH HHS DA010923 / NIDA NIH HHS R01 DA021736 / NIDA NIH HHS R01 DA015789 / NIDA NIH HHS
Language: English
Date published: 03/05/2010
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984003934102771

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