The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro

Liu Hong; Guoquan Zhang; Habiba Sultana; Yang Yu; Zhen Wei

doi:10.1089/scd.2010.0125

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The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro

Journal article

Open access

Peer reviewed

The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro

Liu Hong, Guoquan Zhang, Habiba Sultana, Yang Yu and Zhen Wei

Stem cells and development, Vol.20(5), pp.925-931

05/2011

DOI: 10.1089/scd.2010.0125

PMCID: PMC3128766

PMID: 20735179

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Abstract

Human bone marrow mesenchymal stromal cells (MSCs) with self-renewal and multiple differentiation potentials are considered a possible cell source for tissue engineering and regenerative medicine. However, the limited amount of MSCs in bone marrow and the loss of differentiation capacity following in vitro expansion restrict their practical application. Effective improvement of MSC proliferation is necessary for the clinical application of MSC-based tissue engineering. The effects of estrogen supplements on proliferation and characterizations of human MSCs were investigated at the present study. Supplements of 17-β estradiol (E2) significantly increase the proliferation of human MSCs in vitro. The dose range of E2 to significantly increase MSC proliferation differs in the gender of MSC donor. E2 supplementation in cell proliferation maintains characterizations of MSCs, including cell surface markers, and osteogenic and adipogenic differentiation capacities. These data indicate that estrogen treatment can play an important role in improving human MSCs' expansion in vitro, which will effectively facilitate MSCs' function in the practical application of tissue engineering and regeneration.

Mesenchymal Stromal Cells - drug effects

Tissue Engineering - methods

Bone Marrow Cells - cytology

Adipogenesis - drug effects

Humans

Osteogenesis - drug effects

Biomarkers - analysis

Cells, Cultured

Male

Regenerative Medicine - methods

Mesenchymal Stromal Cells - cytology

Cell Differentiation - drug effects

Sex Factors

Stromal Cells - drug effects

Telomerase - analysis

Adult

Bone Marrow Cells - drug effects

Female

Telomerase - metabolism

Cell Proliferation - drug effects

Cell Culture Techniques

Estradiol - pharmacology

Stromal Cells - cytology

Details

Title: Subtitle: The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro
Creators: Liu Hong - Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, Iowa 52242, USA. liu-hong@uiowa.edu
Guoquan Zhang
Habiba Sultana
Yang Yu
Zhen Wei
Resource Type: Journal article
Publication Details: Stem cells and development, Vol.20(5), pp.925-931
DOI: 10.1089/scd.2010.0125
PMID: 20735179
PMCID: PMC3128766
NLM abbreviation: Stem Cells Dev
ISSN: 1547-3287
eISSN: 1557-8534
Publisher: United States
Grant note: 1R03DE017715 / NIDCR NIH HHS
Language: English
Date published: 05/2011
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Internal Medicine
Record Identifier: 9984065991202771

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