Journal article
The effects of N-acetylcysteine on the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 in hepatic fibrosis in bile duct ligated rats
Hepatology research, Vol.38(12), pp.1252-1263
12/01/2008
DOI: 10.1111/j.1872-034X.2008.00393.x
PMID: 18713279
Abstract
Aim: N-acetylcysteine can inhibit the formation of intracellular reactive oxygen intermediates. Cellular redox state plays a role in regulating the secretion of matrix metalloproteinase-2. We investigated the effects of N-acetylcysteine on the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2.
Methods: Bile duct ligated rats were used as a model of hepatic fibrosis. We compared the level of gene expression (using real-time reverse transcription polymerase chain reaction [RT-PCR]), liver function parameters, hepatic reactive oxygen production, lipid peroxidation and glutathione state in experimental groups.
Results: N-acetylcysteine treatment significantly improved liver function parameters including the plasma levels of aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transpeptidase and bilirubin. In addition, significant improvement of glutathione state and reactive oxygen production were observed. Hepatic lipid peroxidation was reversed by N-acetylcysteine treatment. Although N-acetylcysteine treatment did not completely normalize the increased matrix metalloproteinase-2 expression, it significantly decreased its level by 65%. N-acetylcysteine treatment also significantly decreased matrix metalloproteinase-2 activity and normalized tissue inhibitor of matrix metalloproteinase-2 expression.
Conclusion: Collectively, N-acetylcysteine showed inhibition of matrix metalloproteinase-2 expression and activity. In addition, administration of N-acetylcysteine was associated with downregulation of the expression of tissue inhibitor of matrix metalloproteinase-2 and amelioration of oxidative stress in the liver of bile duct ligated rats.
Details
- Title: Subtitle
- The effects of N-acetylcysteine on the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 in hepatic fibrosis in bile duct ligated rats
- Creators
- Arezou Rezaei - Institute of Biochemistry and Biophysics, Tehran University , Tehran, IranSussan Kaboudanian Ardestani - Institute of Biochemistry and Biophysics, Tehran University,Mehdi Forouzandeh - Tarbiat Modares UniversitySeyed Mohammad Tavangar - Shariati HospitalMohammad Reza Khorramizadeh - Tehran University of Medical SciencesSeyedmehdi Payabvash - Tehran University of Medical SciencesBehtash Ghazi Nezami - Tehran University of Medical SciencesZahra Jahanshiri - Tarbiat Modares UniversityZahra Tavakoli - Institute of Biochemistry and Biophysics, Tehran University,Ahmad Shariftabrizi - Boston UniversityAhmad Reza Dehpour - Tehran University of Medical Sciences
- Resource Type
- Journal article
- Publication Details
- Hepatology research, Vol.38(12), pp.1252-1263
- Publisher
- Wiley
- DOI
- 10.1111/j.1872-034X.2008.00393.x
- PMID
- 18713279
- ISSN
- 1386-6346
- eISSN
- 1872-034X
- Number of pages
- 12
- Grant note
- Faculty of Medicine, Tehran University of Medical Sciences; Tehran University of Medical Sciences Institute of Biochemistry and Biophysics (IBB) Faculty of Science, Tehran University, Tehran, Iran
- Language
- English
- Date published
- 12/01/2008
- Academic Unit
- Radiology
- Record Identifier
- 9984318788402771
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