The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania

Chia-Hung Christine Hsiao; Norikiyo Ueno; Jian Q Shao; Kristin R Schroeder; Kenneth C Moore; John E Donelson; Mary E Wilson

doi:10.1016/j.micinf.2011.05.014

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The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania

Journal article

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The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania

Chia-Hung Christine Hsiao, Norikiyo Ueno, Jian Q Shao, Kristin R Schroeder, Kenneth C Moore, John E Donelson and Mary E Wilson

Microbes and infection, Vol.13(12), pp.1033-1044

2011

DOI: 10.1016/j.micinf.2011.05.014

PMCID: PMC3185139

PMID: 21723411

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https://www.ncbi.nlm.nih.gov/pmc/articles/3185139View

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Abstract

Leishmania spp. protozoa are obligate intracellular parasites that replicate in macrophages during mammalian infection. Efficient phagocytosis and survival in macrophages are important determinants of parasite virulence. Macrophage lines differ dramatically in their ability to sustain intracellular Leishmania infantum chagasi ( Lic). We report that the U937 monocytic cell line supported the intracellular replication and cell-to-cell spread of Lic during 72 h after parasite addition, whereas primary human monocyte-derived macrophages (MDMs) did not. Electron microscopy and live cell imaging illustrated that Lic promastigotes anchored to MDMs via their anterior ends and were engulfed through symmetrical pseudopods. In contrast, U937 cells bound Lic in diverse orientations, and extended membrane lamellae to reorient and internalize parasites through coiling phagocytosis. Lic associated tightly with the parasitophorous vacuole (PV) membrane in both cell types. PVs fused with LAMP-1-expressing compartments 24 h after phagocytosis by MDMs, whereas U937 cell PVs remained LAMP-1 negative. The expression of one phagocytic receptor (CR3) was higher in MDMs than U937 cells, leading us to speculate that parasite uptake proceeds through dissimilar pathways between these cells. We hypothesize that the mechanism of phagocytosis differs between primary versus immortalized human macrophage cells, with corresponding differences in the subsequent intracellular fate of the parasite.

Macrophages

Leishmania

CR3 receptor

U937 cells

Phagocytosis

Details

Title: Subtitle: The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania
Creators: Chia-Hung Christine Hsiao - Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA 52242, USA
Norikiyo Ueno - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Jian Q Shao - Central Microscopy Research Facility, University of Iowa, Iowa City, IA 52242, USA
Kristin R Schroeder - Central Microscopy Research Facility, University of Iowa, Iowa City, IA 52242, USA
Kenneth C Moore - Central Microscopy Research Facility, University of Iowa, Iowa City, IA 52242, USA
John E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Mary E Wilson - Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Microbes and infection, Vol.13(12), pp.1033-1044
DOI: 10.1016/j.micinf.2011.05.014
PMID: 21723411
PMCID: PMC3185139
NLM abbreviation: Microbes Infect
ISSN: 1286-4579
eISSN: 1769-714X
Publisher: Elsevier Masson SAS
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: AI32135, AI059451, AI045540, AI067874, AI080801, AI076233; DOI: 10.13039/100000738, name: Department of Veterans’ Affairs
Language: English
Date published: 2011
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Emergency Medicine; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984001210502771

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