Journal article
The effects of preeclampsia on signaling to hematopoietic progenitor cells
Proceedings in obstetrics and gynecology, Vol.3(1), pp.1-11
04/10/2013
DOI: 10.17077/2154-4751.1207
Abstract
Background: The role of the microenvironment is important in cell differentiation. The effect of placental disease on the growth and differentiation and hematopoietic stem cells has not been well-studied.
Methods: Enzyme linked immunoassay was used to measure erythropoietin and osteopontin in plasma from umbilical cord blood of children born to normotensive and preeclamptic women. Additionally, CD34+ cells were isolated from umbilical cord blood and grown in complete methylcellulose media. Colony types were identified and enumerated.
Results: Differences in the concentration of erythropoietin in the cord blood between the controls and the preeclamptics approached significance (P = 0.067) using a Mann-Whitney U test. In the plasma of cord blood from children born to normotensive women, the median erythropoietin was 0.186 mIU/mL compared to 1.986 mIU/mL in children of preeclamptic women. We did not find any significant differences in the number and types of colonies; however, there was a trend toward increased BFU-E in the preeclamptic samples. Furthermore, this trend for increased BFU-E colonies was also seen from CD34+ cells isolated from umbilical cord blood of severe preeclamptics compared to mild.
Conclusion: Our preliminary studies suggest that abnormalities in the placenta, such as those found when the mother experiences preeclampsia, m
Details
- Title: Subtitle
- The effects of preeclampsia on signaling to hematopoietic progenitor cells
- Creators
- Donna A. Santillan - University of IowaWendy S. Hamilton - University of IowaAshley Christensen - Roy J. and Lucille A. Carver College of MedicineKatelyn M. Talcott - University of IowaLindsay K. Gravatt - University of IowaMark K. Santillan - University of IowaStephen K. Hunter - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Proceedings in obstetrics and gynecology, Vol.3(1), pp.1-11
- DOI
- 10.17077/2154-4751.1207
- ISSN
- 2154-4751
- eISSN
- 2154-4751
- Language
- English
- Date published
- 04/10/2013
- Academic Unit
- Obstetrics and Gynecology; Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984317121102771
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