Journal article
The efficient intracellular sequestration of the insulin-regulatable glucose transporter (GLUT-4) is conferred by the NH2 terminus
The Journal of cell biology, Vol.117(4), pp.729-743
05/1992
DOI: 10.1083/jcb.117.4.729
PMCID: PMC2289457
PMID: 1577853
Abstract
GLUT-4 is the major facilitative glucose transporter isoform in tissues that exhibit insulin-stimulated glucose transport. Insulin regulates glucose transport by the rapid translocation of GLUT-4 from an intracellular compartment to the plasma membrane. A critical feature of this process is the efficient exclusion of GLUT-4 from the plasma membrane in the absence of insulin. To identify the amino acid domains of GLUT-4 which confer intracellular sequestration, we analyzed the subcellular distribution of chimeric glucose transporters comprised of GLUT-4 and a homologous isoform, GLUT-1, which is found predominantly at the cell surface. These chimeric transporters were transiently expressed in CHO cells using a double subgenomic recombinant Sindbis virus vector. We have found that wild-type GLUT-4 is targeted to an intracellular compartment in CHO cells which is morphologically similar to that observed in adipocytes and muscle cells. Sindbis virus-produced GLUT-1 was predominantly expressed at the cell surface. Substitution of the GLUT-4 amino-terminal region with that of GLUT-1 abolished the efficient intracellular sequestration of GLUT-4. Conversely, substitution of the NH2 terminus of GLUT-1 with that of GLUT-4 resulted in marked intracellular sequestration of GLUT-1. These data indicate that the NH2-terminus of GLUT-4 is both necessary and sufficient for intracellular sequestration.
Details
- Title: Subtitle
- The efficient intracellular sequestration of the insulin-regulatable glucose transporter (GLUT-4) is conferred by the NH2 terminus
- Creators
- R C Piper - Washington University in St. LouisC Tai - Washington University in St. LouisJ W Slot - Washington University in St. LouisC S Hahn - Washington University in St. LouisC M Rice - Washington University in St. LouisH Huang - Washington University in St. LouisD E James - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.117(4), pp.729-743
- DOI
- 10.1083/jcb.117.4.729
- PMID
- 1577853
- PMCID
- PMC2289457
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Grant note
- DK42503 / NIDDK NIH HHS
- Language
- English
- Date published
- 05/1992
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297600602771
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