Journal article
The emerging contaminant 3,3 '-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)
ENVIRONMENTAL POLLUTION, Vol.254 Part A, 113027
2019
DOI: 10.1016/j.envpol.2019.113027
PMCID: PMC7027435
PMID: 31421573
Abstract
3,3'-dichlorobiphenyl (PCB-11) is an emerging PCB congener widely detected in environmental samples and human serum, but its toxicity potential is poorly understood. We assessed the effects of three concentrations of PCB-11 on embryotoxicity and Aryl hydrocarbon receptor (Ahr) pathway interactions in zebrafish embryos (Danio rerio). Wildtype AB or transgenic Tg(gut:GFP) strain zebrafish embryos were exposed to static concentrations of PCB-11 (0, 0.2, 2, or 20 mu M) from 24 to 96 h post fertilization (hpf), and gross morphology, Cytochrome P4501a (Cypl a) activity, and liver development were assessed via microscopy. Ahr interactions were probed via co-exposures with PCB-126 or beta-naphthoflavone (BNF). Embryos exposed to 20 mu M PCB-11 were also collected for PCB-11 body burden, qRT-PCR, RNAseq, and histology. Zebrafish exposed to 20 mu M PCB-11 absorbed 0.18% PCB-11 per embryo at 28 hpf and 0.61% by 96 hpf, and their media retained 136% PCB-11 at 28 hpf and 0.84% at 96 hpf. This concentration did not affect gross morphology, but altered the transcription of xenobiotic metabolism and liver development genes, impeded liver development, and increased hepatocyte vacuole formation. In co-exposures, 20 mu M PCB-11 prevented deformities caused by PCB-126 but exacerbated deformities in co-exposures with BNF. This study suggests that PCB-11 can affect liver development, act as a partial agonist/antagonist of the Ahr pathway, and act as an antagonist of Cypl a activity to modify the toxicity of compounds that interact with the Ahr pathway. (C) 2019 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- The emerging contaminant 3,3 '-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)
- Creators
- Monika A Roy - University of Massachusetts AmherstKarilyn E Sant - University of Massachusetts AmherstOlivia L Venezia - University of Massachusetts AmherstAlix B Shipman - University of Massachusetts AmherstStephen D McCormick - United States Geological SurveyPanithi Saktrakulkla - University of IowaKeri C Hornbuckle - University of IowaAlicia R Timme-Laragy - University of Massachusetts Amherst
- Resource Type
- Journal article
- Publication Details
- ENVIRONMENTAL POLLUTION, Vol.254 Part A, 113027
- DOI
- 10.1016/j.envpol.2019.113027
- PMID
- 31421573
- PMCID
- PMC7027435
- NLM abbreviation
- Environ Pollut
- ISSN
- 1873-6424
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01ES025748, F32ES028085; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P42ES013661; DOI: 10.13039/100008975, name: University of Massachusetts Amherst; DOI: 10.13039/100011495, name: Massachusetts Life Sciences Center
- Language
- English
- Date published
- 2019
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; IIHR--Hydroscience and Engineering; Interdisciplinary Graduate Program in Human Toxicology; Iowa Superfund Research Program; Interdisciplinary Programs
- Record Identifier
- 9984231892502771
Metrics
17 Record Views