Journal article
The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or ‘Ly 1-like’ B cells
International immunology, Vol.3(4), pp.305-315
1991
DOI: 10.1093/intimm/3.4.305
PMID: 1831650
Abstract
The distribution of IgE FcR (Fc epsilon R)-positive and -negative B cells was examined in normal adult mice. Using three-color flow cytometry, the expression of the Fc epsilon R was analyzed on various B-cell subsets present in the peritoneum and spleen. The results demonstrate that in the peritoneal cavity, the Fc epsilon R is not expressed on the large majority of Ly 1+ B cells and Ly 1-, Mac 1+ sister B cells. The receptor is present, however, on the small number of conventional B cells residing in the peritoneum. Although interleukin 4 (IL-4) can increase the levels of the Fc epsilon R on conventional B cells, incubation of Ly 1 and sister B cells with IL-4 did not result in the expression of the Fc epsilon R. When examining B cells present in the spleen, a small subset of B cells was consistently found to be Fc epsilon R-. These Fc epsilon R- cells were IgM-bright, IgD-dull and largely Ly 1- and Mac 1-negative. Staining of splenic tissue sections revealed that the Fc epsilon R- B cells were primarily localized to the marginal zones, whereas the Fc epsilon R+ B cells were found in the follicles. Taken together, the results indicate that the Fc epsilon R may be a useful marker in delineating the various B-cell subsets. In the peritoneum, the Fc epsilon R appears to discriminate conventional B cells from those of the Ly 1/sister lineage, and in the spleen it is likely to distinguish resting follicular B cells from Ly 1/sister and marginal zone B cells.
Details
- Title: Subtitle
- The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or ‘Ly 1-like’ B cells
- Creators
- Thomas J WaldschmidtFrans G. M KroeseLorraine T TygrettDaniel H ConradRichard G Lynch
- Resource Type
- Journal article
- Publication Details
- International immunology, Vol.3(4), pp.305-315
- DOI
- 10.1093/intimm/3.4.305
- PMID
- 1831650
- ISSN
- 0953-8178
- eISSN
- 1460-2377
- Language
- English
- Date published
- 1991
- Academic Unit
- Pathology
- Record Identifier
- 9984047869602771
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