Journal article
The generation of a novel spontaneous transgenic mouse model of node positive metastatic vulvar cancer
Gynecologic oncology, Vol.131(1), pp.261-261
10/2013
DOI: 10.1016/j.ygyno.2013.07.038
Abstract
Very little is known about the pathophysiology of vulvar cancer. Approximately 4000 individuals are diagnosed with the disease each year and almost 1000 deaths are attributable to the disease annually. Treatment often involves surgical resection, which can be very disfiguring. Once metastases have occurred, conventional radiation and chemotherapy are used in attempt to slow disease progression. Given its low incidence, it is difficult to identify and characterize genes associated with the development of vulvar cancer. The Sleeping Beauty (SB) insertional mutagenesis system consists of a transposable element (a transposon) and an activating enzyme (atransposase) that induces random translocation of the transposon from one DNA site to another. The introduction of the transposon disrupts DNA, resulting in inactivation of tumor suppressors or activation of oncogenes. Mutations that enhance unregulated cellular survival and proliferation are selected for, resulting in the development of cancer. The SB insertional mutagenesis system allows for tracking of tumor-driving mutations. The purpose of our project was to develop a mouse model of vulvar cancer utilizing Sleeping Beauty (SB) insertional mutagenesis to identify novel genetic mutations driving vulvar cancer development.
Details
- Title: Subtitle
- The generation of a novel spontaneous transgenic mouse model of node positive metastatic vulvar cancer
- Creators
- A. Guimarães YoungT.L NeffM SamuelsonD.K MeyerholzA.J DupuyM.J Goodheart
- Resource Type
- Journal article
- Publication Details
- Gynecologic oncology, Vol.131(1), pp.261-261
- DOI
- 10.1016/j.ygyno.2013.07.038
- ISSN
- 0090-8258
- eISSN
- 1095-6859
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Pathology; Obstetrics and Gynecology; Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984047665002771
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