The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology

Timothy E Mattes; Anne K Alexander; Paul M Richardson; A Christine Munk; Cliff S Han; Paul Stothard; Nicholas V Coleman

doi:10.1128/AEM.00197-08

Back

The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology

Journal article

Open access

Peer reviewed

The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology

Timothy E Mattes, Anne K Alexander, Paul M Richardson, A Christine Munk, Cliff S Han, Paul Stothard and Nicholas V Coleman

Applied and environmental microbiology, Vol.74(20), pp.6405-6416

10/2008

DOI: 10.1128/AEM.00197-08

PMCID: PMC2570305

PMID: 18723656

Files and links (1)

url

https://doi.org/10.1128/AEM.00197-08View

Published (Version of record) Open Access

Abstract

Polaromonas sp. strain JS666 can grow on cis-1,2-dichloroethene (cDCE) as a sole carbon and energy source and may be useful for bioremediation of chlorinated solvent-contaminated sites. Analysis of the genome sequence of JS666 (5.9 Mb) shows a bacterium well adapted to pollution that carries many genes likely to be involved in hydrocarbon and xenobiotic catabolism and metal resistance. Clusters of genes coding for haloalkane, haloalkanoate, n-alkane, alicyclic acid, cyclic alcohol, and aromatic catabolism were analyzed in detail, and growth on acetate, catechol, chloroacetate, cyclohexane carboxylate, cyclohexanol, ferulate, heptane, 3-hydroxybenzoate, hydroxyquinol, gentisate, octane, protocatechuate, and salicylate was confirmed experimentally. Strain JS666 also harbors diverse putative mobile genetic elements, including retrons, inteins, a miniature inverted-repeat transposable element, insertion sequence transposases from 14 families, eight genomic islands, a Mu family bacteriophage, and two large (338- and 360-kb) plasmids. Both plasmids are likely to be self-transferable and carry genes for alkane, alcohol, aromatic, and haloacid metabolism. Overall, the JS666 genome sequence provides insights into the evolution of pollutant-degrading bacteria and provides a toolbox of catabolic genes with utility for biotechnology.

Genes, Bacterial

Genome, Bacterial

Multigene Family

Comamonadaceae - genetics

Interspersed Repetitive Sequences

Bacterial Proteins - genetics

Hydrocarbons - metabolism

Molecular Sequence Data

DNA, Bacterial - chemistry

Sequence Analysis, DNA

DNA, Bacterial - genetics

Enzymes - genetics

Xenobiotics - metabolism

Evolution, Molecular

Details

Title: Subtitle: The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology
Creators: Timothy E Mattes - Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, Iowa 52242, USA. tim-mattes@uiowa.edu
Anne K Alexander
Paul M Richardson
A Christine Munk
Cliff S Han
Paul Stothard
Nicholas V Coleman
Resource Type: Journal article
Publication Details: Applied and environmental microbiology, Vol.74(20), pp.6405-6416
DOI: 10.1128/AEM.00197-08
PMID: 18723656
PMCID: PMC2570305
NLM abbreviation: Appl Environ Microbiol
ISSN: 0099-2240
eISSN: 1098-5336
Publisher: United States
Language: English
Date published: 10/2008
Academic Unit: Civil and Environmental Engineering
Record Identifier: 9983991965302771

Metrics

15 Record Views

114 Times Cited - Web of Science