Journal article
The genome-wide DNA methylation profiles among neurosurgery patients with and without post-operative delirium
Psychiatry and clinical neurosciences, Vol.77(1), pp.48-55
01/2023
DOI: 10.1111/pcn.13495
PMCID: PMC9812874
PMID: 36266784
Appears in UI Libraries Support Open Access
Abstract
AIMSThere is no previous study demonstrating the differences of genome-wide DNA methylation (DNAm) profiles between patients with and without postoperative delirium (POD). We aimed to discover epigenetic (DNAm) markers that are associated with POD in blood obtained from patients before and after neurosurgery. METHODSPre- and post-surgical blood DNA samples from 37 patients, including 10 POD cases, were analyzed using the Illumina EPIC array genome-wide platform. We examined DNAm differences in blood from patients with and without POD. Enrichment analysis with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms were also conducted. RESULTSWhen POD cases were tested for DNAm change before and after surgery, enrichment analyses showed many relevant signals with statistical significance in immune response related-pathways and inflammatory cytokine related-pathways such as "cellular response to cytokine stimulus", "regulation of immune system process", "regulation of cell activation", and "regulation of cytokine production". Furthermore, after excluding the potential effect of common factors related to surgery and anesthesia between POD cases and non-POD controls, the enrichment analyses showed significant signals such as "immune response" and "T cell activation", which are same pathways previously identified from an independent non-surgical inpatient cohort. CONCLUSIONSOur first genome-wide DNAm investigation of POD showed promising signals related to immune response, inflammatory response and other relevant signals considered to be associated with delirium pathophysiology. Our data supports the hypothesis that epigenetics are playing an important role in pathophysiological mechanism of delirium and suggest the potential usefulness of epigenetics based biomarker of POD. This article is protected by copyright. All rights reserved.
Details
- Title: Subtitle
- The genome-wide DNA methylation profiles among neurosurgery patients with and without post-operative delirium
- Creators
- Takehiko Yamanashi - Stanford UniversityKaitlyn J Crutchley - University of Nebraska Medical CenterNadia E Wahba - Roy J. and Lucille A. Carver College of MedicineTakaaki Nagao - Roy J. and Lucille A. Carver College of MedicinePedro S Marra - Roy J. and Lucille A. Carver College of MedicineCade C Akers - Roy J. and Lucille A. Carver College of MedicineEleanor J Sullivan - Roy J. and Lucille A. Carver College of MedicineMasaaki Iwata - Tottori UniversityMathew A Howard - Roy J. and Lucille A. Carver College of MedicineHyunkeun R Cho - University of IowaHiroto Kawasaki - Roy J. and Lucille A. Carver College of MedicineChristopher G Hughes - Vanderbilt University Medical CenterPratik P Pandharipande - Vanderbilt University Medical CenterMarco M Hefti - Roy J. and Lucille A. Carver College of MedicineGen Shinozaki - Stanford University
- Resource Type
- Journal article
- Publication Details
- Psychiatry and clinical neurosciences, Vol.77(1), pp.48-55
- DOI
- 10.1111/pcn.13495
- PMID
- 36266784
- PMCID
- PMC9812874
- NLM abbreviation
- Psychiatry Clin Neurosci
- eISSN
- 1440-1819
- Publisher
- Wiley
- Language
- English
- Electronic publication date
- 10/20/2022
- Date published
- 01/2023
- Academic Unit
- Neurology; Psychiatry; Pathology; Iowa Neuroscience Institute; Biostatistics; Neurosurgery; Otolaryngology
- Record Identifier
- 9984306660102771
Metrics
20 Record Views