Journal article
The glycosphingolipid composition of the placenta of a blood group P fetus delivered by a blood group Pk1 woman and analysis of the anti-globoside antibodies found in maternal serum
Archives of biochemistry and biophysics, Vol.260(1), pp.168-176
01/1988
DOI: 10.1016/0003-9861(88)90438-9
PMID: 3341739
Abstract
To further define the molecules that may mediate spontaneous abortion due to maternal-fetal blood group incompatibility within the P blood group system, we have examined the fine specificities of maternal antibodies and the glycolipid antigens from the placenta of a P infant born to a Pk1 mother. Maternal antibodies obtained during therapeutic plasmapheresis were analyzed to determine their reactivities with placental glycolipid extracts on thin-layer plates. Second antibodies specific for IgM, IgG, and IgA revealed immunoglobulins of all of these classes strongly reactive with one major placental glycolipid that comigrates with globoside. GC/MS analysis confirmed that the major P-active pentaglycosylceramide of placenta has the same structure as that previously shown for the P antigen of red blood cells: GalNAcβ13Galαl4Galβ14GlcCer. Serum antibodies partially purified by affinity chromatography on globoside-octyl-Sepharose specifically recognize glycolipids that contain terminal GalNAcβ1-3Gal … residues and also recognize the same sequence as an internal determinant in some, but not all, glycolipids with extended globoside core regions. Thus, in the blood group P incompatible fetus, the major P antigen present in placenta has the same carbohydrate structure as the P antigen present in fetal and adult erythrocytes and might be a target for the maternal immune system.
Details
- Title: Subtitle
- The glycosphingolipid composition of the placenta of a blood group P fetus delivered by a blood group Pk1 woman and analysis of the anti-globoside antibodies found in maternal serum
- Creators
- Gunnar C. HanssonKazimiera WazniowskaJohn A. RockPaul M. NessThomas S. KicklerRosetta S. ShireyJennifer R. Niebyl - University of Iowa, Obstetrics and GynecologyDavid Zopf
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.260(1), pp.168-176
- DOI
- 10.1016/0003-9861(88)90438-9
- PMID
- 3341739
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 01/1988
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557422402771
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