Journal article
The herpes simplex virus 1 RNA binding protein US11 is a virion component and associates with ribosomal 60S subunits
Journal of virology, Vol.66(6), pp.3624-3632
06/1992
DOI: 10.1128/JVI.66.6.3624-3632.1992
PMCID: PMC241145
PMID: 1316472
Abstract
The herpes simplex virus 1 US11 gene encodes a site- and conformation-specific RNA binding regulatory protein. We fused the coding sequence of this protein with that of beta-galactosidase, expressed the chimeric gene in Escherichia coli, and purified a fusion protein which binds RNA in the same way as the infected cell protein. The fusion protein was used to generate anti-US11 monoclonal antibody. Studies with this antibody showed that US11 protein is a viral structural protein estimated to be present in 600 to 1,000 copies per virion. The great majority of cytoplasmic US11 protein was found in association with the 60S subunit of infected cell ribosomes. US11 protein associates with ribosomes both late in infection at the time of its synthesis and at the time of infection after its introduction into the cytoplasm by the virion. US11 protein expressed in an uninfected cell line stably transfected with the US11 gene associates with ribosomal 60S subunits and localizes to nucleoli, suggesting that US11 protein requires no other viral functions for these associations.
Details
- Title: Subtitle
- The herpes simplex virus 1 RNA binding protein US11 is a virion component and associates with ribosomal 60S subunits
- Creators
- R J Roller - Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637B Roizman - Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.66(6), pp.3624-3632
- DOI
- 10.1128/JVI.66.6.3624-3632.1992
- PMID
- 1316472
- PMCID
- PMC241145
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 06/1992
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001118302771
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