The herpes simplex virus type 1 ribonucleotide reductase is required for acute retinal disease

C R Brandt; P Imesch; B Spencer; B Eliassi-Rad; N A Syed; S Untawale; N L Robinson; D M Albert

doi:10.1007/s007050050126

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The herpes simplex virus type 1 ribonucleotide reductase is required for acute retinal disease

Journal article

Peer reviewed

The herpes simplex virus type 1 ribonucleotide reductase is required for acute retinal disease

C R Brandt, P Imesch, B Spencer, B Eliassi-Rad, N A Syed, S Untawale, N L Robinson and D M Albert

Archives of virology, Vol.142(5), pp.883-896

1997

DOI: 10.1007/s007050050126

PMID: 9191855

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Abstract

We have used a herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR) null mutant (ICP6 delta) to determine if the HSV-1 RR is required for acute retinal disease. Injection of the ICP6 delta mutant into the vitreous induced mild transient signs of infection (vitreal infiltrate, retinal inflammation, and changes in retinal cytology). In contrast, the parental KOS and a revertant virus (ICP6 delta + 3.1) in which the RR gene had been restored, caused severe retinitis. Injection of media alone also induced mild transient signs of disease. Two months after infection, ICP6 delta injected eyes could not be distinguished from normal eyes. Repeated injection of ICP6 delta (3 times, 2 weeks apart) resulted in vitreal infiltrate near the site of injection but the retina did not appear damaged. The mutant, ICP6 delta, grew to peak titers 1 x 10(3) to 1 x 10(5)-fold lower and cleared faster than KOS or ICP6 delta + 3.1 in the injected eyes suggesting that the reduced virulence was due to reduced ability of the virus to grow. These results show that the viral RR is required for acute retinal disease.

Immunohistochemistry

Retinitis - pathology

Acute Disease

Antigens, Viral - analysis

Virulence

Male

Mice, Inbred Strains

Animals

Ribonucleotide Reductases - analysis

Herpesvirus 1, Human - enzymology

Retinitis - virology

Virus Replication

Female

Herpesvirus 1, Human - genetics

Mice

Ribonucleotide Reductases - genetics

Mutation

Herpesvirus 1, Human - pathogenicity

Details

Title: Subtitle: The herpes simplex virus type 1 ribonucleotide reductase is required for acute retinal disease
Creators: C R Brandt - Department of Ophthalmology, University of Wisconsin Medical School, Madison, USA
P Imesch
B Spencer
B Eliassi-Rad
N A Syed
S Untawale
N L Robinson
D M Albert
Resource Type: Journal article
Publication Details: Archives of virology, Vol.142(5), pp.883-896
Publisher: Austria
DOI: 10.1007/s007050050126
PMID: 9191855
ISSN: 0304-8608
eISSN: 1432-8798
Grant note: EY07736 / NEI NIH HHS EY01917 / NEI NIH HHS SP3OCA14520 / CSAP SAMHSA HHS
Language: English
Date published: 1997
Academic Unit: Pathology; Ophthalmology and Visual Sciences
Record Identifier: 9983980398002771

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