Journal article
The immediate early genes of human cytomegalovirus upregulate tumor necrosis factor-alpha gene expression
The Journal of clinical investigation, Vol.93(2), pp.474-478
02/1994
DOI: 10.1172/JCI116995
PMCID: PMC293860
PMID: 8113386
Abstract
Cytomegalovirus (CMV) is an important cause of disease in the immunocompromised patient and CMV infection is associated with predominantly mononuclear inflammatory response. Since products of the CMV immediate early (IE) gene region are potent trans-activators, we used the monocyte cell line THP-1 and a transient transfection assay to determine if these viral proteins upregulate expression of the TNF gene. The IE genes of CMV upregulated TNF gene activity as judged by increases in promoter activity, steady state mRNA, and protein production. The presence or absence of the 3' untranslated region of the TNF gene did not affect gene expression induced by the IE gene products. These studies suggest that activation of TNF gene expression by the CMV IE gene products may, in part, account for the inflammatory response associated with CMV infections.
Details
- Title: Subtitle
- The immediate early genes of human cytomegalovirus upregulate tumor necrosis factor-alpha gene expression
- Creators
- Lois J Geist - Department of Medicine, Department of Veterans Affairs, Iowa City, IowaMartha M Monick - Department of Medicine, Department of Veterans Affairs, Iowa City, IowaMark F Stinski - Department of Medicine, Department of Veterans Affairs, Iowa City, IowaGary W Hunninghake - Department of Medicine, Department of Veterans Affairs, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of clinical investigation, Vol.93(2), pp.474-478
- DOI
- 10.1172/JCI116995
- PMID
- 8113386
- PMCID
- PMC293860
- ISSN
- 0021-9738
- eISSN
- 1558-8238
- Language
- English
- Date published
- 02/1994
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Provost Office Administration; Internal Medicine
- Record Identifier
- 9984213447202771
Metrics
5 Record Views