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The impact of pre-existing memory on differentiation of newly recruited naive CD8 T cells
Journal article   Peer reviewed

The impact of pre-existing memory on differentiation of newly recruited naive CD8 T cells

Matthew D Martin, Thomas C Wirth, Peter Lauer, John T Harty and Vladimir P Badovinac
The Journal of immunology (1950), Vol.187(6), pp.2923-2931
09/15/2011
DOI: 10.4049/jimmunol.1100698
PMCID: PMC3169750
PMID: 21832161

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Abstract

One goal of immunization is to generate memory CD8 T cells of sufficient quality and quantity to confer protection against infection. It has been shown that memory CD8 T cell differentiation in vivo is controlled, at least in part, by the amount and duration of infection, Ag, and inflammatory cytokines present early after the initiation of the response. In this study, we used models of anti-vectorial immunity to investigate the impact of pre-existing immunity on the development and differentiation of vector-induced primary CD8 T cell responses. We showed that existing CD8 T cell memory influences the magnitude of naive CD8 T cell responses. However, the differentiation of newly recruited (either TCR-transgenic or endogenous) primary CD8 T cells into populations with the phenotype (CD62L(hi), CD27(hi), KLRG-1(low)) and function (tissue distribution, Ag-driven proliferation, cytokine production) of long-term memory was facilitated when they were primed in the presence of vector-specific memory CD8 T cells of the same or unrelated specificity. Therefore, these data suggested that the presence of anti-vectorial immunity impacts the rate of differentiation of vector-induced naive CD8 T cells, a notion with important implications for the design of future vaccination strategies.
CD8-Positive T-Lymphocytes - cytology Mice, Inbred C57BL Immunophenotyping Mice, Transgenic Adoptive Transfer Cell Differentiation - immunology Lymphocyte Activation - immunology Phenotype Animals Mice CD8-Positive T-Lymphocytes - immunology Cytokines - biosynthesis Immunologic Memory - immunology Cytokines - immunology

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