The in vivo cross-linking of proteins and DNA by heavy metals

A WEDRYCHOWSKI; Warren N Schmidt; Lubomir S Hnilica

doi:10.1016/S0021-9258(17)35792-7

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The in vivo cross-linking of proteins and DNA by heavy metals

Journal article

Open access

Peer reviewed

The in vivo cross-linking of proteins and DNA by heavy metals

A WEDRYCHOWSKI, Warren N Schmidt and Lubomir S Hnilica

The Journal of biological chemistry, Vol.261(7), pp.3370-3376

1986

DOI: 10.1016/S0021-9258(17)35792-7

PMID: 3512554

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Abstract

Cross-linking of proteins to DNA in live, intact Novikoff ascites hepatoma cells exposed in vitro to different concentrations of CuSO4, Pb(NO3)2, HgCl2, and AlCl3 was studied. Protein-DNA complexes were separated by high-speed centrifugation of cells solubilized in buffered 4% sodium dodecyl sulfate and assayed by electrophoretic separation of proteins associated with the DNA-containing pellets. Concentration dependence experiments showed that the optimal cross-linking occurred at metal concentration of 0.5 mM for CuSO4, HgCl2, and AlCl3 while the optimal cross-linking for Pb(NO3)2 was at 5 mM. For some metals at concentrations higher than optimal, the amounts of cross-linked proteins decreased significantly. Immunochemical analysis of the cross-linked proteins using antibodies to matrix, chromatin, lamins, and cytokeratin fractions demonstrated that some, but not all, members of these protein families became cross-linked to the DNA. Each metal exhibited a cross-linking pattern of its own, different from those of the other metals. Radioactive labeling experiments showed that all the metals tested became associated with the DNA-protein pellets within 1 h after their addition to the incubation medium. However, hexavalent chromium required more than 2 h before appearing in the DNA-protein pellets in significant amounts.

Fundamental and applied biological sciences. Psychology

Analytical biochemistry: general aspects, technics, instrumentation

Biological and medical sciences

Analytical, structural and metabolic biochemistry

Details

Title: Subtitle: The in vivo cross-linking of proteins and DNA by heavy metals
Creators: A WEDRYCHOWSKI - Vanderbilt univ., school medicine
Warren N Schmidt - Vanderbilt univ., school medicine
Lubomir S Hnilica - Vanderbilt univ., school medicine
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.261(7), pp.3370-3376
DOI: 10.1016/S0021-9258(17)35792-7
PMID: 3512554
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
Language: English
Date published: 1986
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094316702771

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