Logo image
Back
The influence of mitochondrial KATP-channels in the cardioprotection of preconditioning and postconditioning by sevoflurane in the rat in vivo
Journal article   Open access   Peer reviewed

The influence of mitochondrial KATP-channels in the cardioprotection of preconditioning and postconditioning by sevoflurane in the rat in vivo

Detlef Obal, Saskia Dettwiler, Christian Favoccia, Horst Scharbatke, Benedikt Preckel and Wolfgang Schlack
Anesthesia and analgesia, Vol.101(5), pp.1252-1260
11/2005
DOI: 10.1213/01.ane.0000181336.96511.32
PMID: 16243977
url
https://doi.org/10.1213/01.ane.0000181336.96511.32View
Published (Version of record) Open Access

Abstract

<p>Volatile anesthetics induce myocardial preconditioning and can also protect the heart when given at the onset of reperfusion - a practice recently termed "postconditioning." We investigated the role of mitochondrial K-ATP (mK(ATP))-channels in sevoflurane-induced cardioprotection for both preconditioning and postconditioning alone and whether there is a synergistic effect of both. Rats were subjected to 25 min of coronary artery occlusion followed by 120 min of reperfusion. Infarct size was determined by triphenyltetrazolium staining. The following protocols were used: 1) preconditioning (S-Pre, n = 10, achieved by 2 periods of 5 min sevoflurane administration (I MAC followed by 10 min of washout); 2) sevoflurane postconditioning (I MAC of sevoflurane given for 2 min at the beginning of reperfusion; S-Post, n = 10); 3) administration before and after ischemia (S-Pre + S-Post, n = 10). Protocols 1-3 were repeated in the presence of 5-hydroxydecanoate (5HD), a specific mK(ATP)-Channel-blocker (S-Pre + S-Post + 5HD, S-Pre + 5HD: n = 10; S-Post + 5HD: n = 9). Nine rats served as untreated controls (CON) or received 5HD alone (5HD, n = 10). Both S-Pre (23% +/- 13% of the area at risk, mean +/- SD) and S-Post (18% +/- 5%) reduced infarct size compared with CON (49% +/- 11%, both P < 0.05). S-Pre + S-Post resulted in a larger reduction of infarct size (12% +/- 5%, P = 0.054 versus S-Pre) compared with administration before or after ischemia alone. 5HD diminished the protection in all three sevoflurane treated groups (S-Pre + 5HD, 35% +/- 12%; S-Post + 5HD, 44% +/- 12%; S-Pre + S-Post + 5HD, 46% +/- 14%;) but given alone had no effect on infarct size (41% +/- 13%). Sevoflurane preconditioning and postconditioning protects against myocardial ischemia-reperfusion injury. The combination of preconditioning and postconditioning provides additive cardioprotection and is mediated, at least in part, by mK(ATP)-channels.</p>
 Anesthesia Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Medical sciences

Details

Metrics

7 Record Views
148 Times Cited - Web of Science
Logo image