The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green

Jinfu Zhao; Bryan A Bertoglio; Michael J Devinney Jr; Kirk E Dineley; Alan R Kay

doi:10.1016/j.ab.2008.09.019

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The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green

Journal article

Peer reviewed

The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green

Jinfu Zhao, Bryan A Bertoglio, Michael J Devinney Jr, Kirk E Dineley and Alan R Kay

Analytical biochemistry, Vol.384(1), pp.34-41

2009

DOI: 10.1016/j.ab.2008.09.019

PMCID: PMC2630397

PMID: 18848515

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http://doi.org/10.1016/j.ab.2008.09.019View

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Abstract

Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells.

Fluorescence

Iron

Zinc

Cadmium

Nickel

Copper

Cobalt

Manganese

Details

Title: Subtitle: The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green
Creators: Jinfu Zhao - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA
Bryan A Bertoglio - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA
Michael J Devinney Jr - University of Pittsburgh
Kirk E Dineley - Department of Biology, Francis Marion University, Florence, SC 29501, USA
Alan R Kay - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Analytical biochemistry, Vol.384(1), pp.34-41
DOI: 10.1016/j.ab.2008.09.019
PMID: 18848515
PMCID: PMC2630397
NLM abbreviation: Anal Biochem
ISSN: 0003-2697
eISSN: 1096-0309
Publisher: Elsevier Inc
Language: English
Date published: 2009
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983992062002771

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