Journal article
The large-scale digital cell analysis system: an open system for nonperturbing live cell imaging
Journal of microscopy (Oxford), Vol.228(Pt 3), pp.296-308
12/2007
DOI: 10.1111/j.1365-2818.2007.01847.x
PMID: 18045324
Abstract
The Large-Scale Digital Cell Analysis System (LSDCAS) was designed to provide a highly extensible open source live cell imaging system. Analysis of cell growth data has demonstrated a lack of perturbation in cells imaged using LSDCAS, through reference to cell growth data from cells growing in CO(2) incubators. LSDCAS consists of data acquisition, data management and data analysis software, and is currently a Core research facility at the Holden Comprehensive Cancer Center at the University of Iowa. Using LSDCAS analysis software, this report and others show that although phase-contrast imaging has no apparent effect on cell growth kinetics and viability, fluorescent image acquisition in the cell lines tested caused a measurable level of growth perturbation using LSDCAS. This report describes the current design of the system, reasons for the implemented design, and details its basic functionality. The LSDCAS software runs on the GNU/Linux operating system, and provides easy to use, graphical programs for data acquisition and quantitative analysis of cells imaged with phase-contrast or fluorescence microscopy (alone or in combination), and complete source code is freely available under the terms of the GNU Public Software License at the project website (http://lsdcas.engineering.uiowa.edu).
Details
- Title: Subtitle
- The large-scale digital cell analysis system: an open system for nonperturbing live cell imaging
- Creators
- Paul J Davis - Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USAElizabeth A KosmacekYuansheng SunFiorenza IanziniMichael A Mackey
- Resource Type
- Journal article
- Publication Details
- Journal of microscopy (Oxford), Vol.228(Pt 3), pp.296-308
- Publisher
- England
- DOI
- 10.1111/j.1365-2818.2007.01847.x
- PMID
- 18045324
- ISSN
- 0022-2720
- eISSN
- 1365-2818
- Grant note
- CA84801 / NCI NIH HHS CA86862 / NCI NIH HHS
- Language
- English
- Date published
- 12/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology
- Record Identifier
- 9984064579202771
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