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The lazaroid U74389G protects normal brain from stereotactic radiosurgery-induced radiation injury
Journal article   Peer reviewed

The lazaroid U74389G protects normal brain from stereotactic radiosurgery-induced radiation injury

John M Buatti, William A Friedman, Daniel P Theele, Francis J Bova and William M Mendenhall
International journal of radiation oncology, biology, physics, Vol.34(3), pp.591-597
1996
DOI: 10.1016/0360-3016(95)02113-2
PMID: 8621283

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Abstract

Purpose : To test an established model of stereotactic radiosurgery-induced radiation injury with pretreatments of either methylprednisolone or the lazaroid U74389G. Methods and Materials : Nine cats received stereotactic radiosurgery with a linear accelerator using an animal radiosurgery device. Each received a dose of 125.0 Gy prescribed to the 84% isodose shell to the anterior limb of the right internal capsule. One animal received no pretreatment, two received citrate vehicle, three received 30 mg/kg of methylprednisolone, and three received 5 mg/kg of U74389G. After irradiation, the animals had frequent neurologic examinations, and neurologic deficits developed in all of them. Six months after the radiation treatment, the animals were anesthetized, and had gadolinium-enhanced magnetic resonance (MR) scans, followed by Evans blue dye perfusion, euthanasia, and brain fixation. Results : Magnetic resonance scans revealed a decrease in the size of the lesions from a mean volume of 0.45 ± 0.06 cm 3 in the control, vehicle-treated, and methylprednisolone-treated animals to 0.22 ± 0.14 cm 3 in the U74389G-treated group. The scans also suggested the absence of necrosis and ventricular dilatation in the lazaroid-treated group. Gross pathology revealed that lesions produced in the untreated, vehicle-treated, and methylprednisolone-treated cats were similar and were characterized by a peripheral zone of Evans blue dye staining with a central zone of a mature coagulative necrosis and focal hemorrhage. However, in the U74389G-treated animals, the lesions were found to have an area of Evans blue dye staining, but lacked discrete areas of necrosis and hemorrhage. Conclusion : These results suggest that the lazaroid U74389G protects the normal brain from radiation injury produced by stereotactic radiosurgery.
Radiation injuries Methylprednisolone Radiation protection Radiosurgery

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