Journal article
The lipid droplet protein Jabba promotes actin remodeling downstream of prostaglandin signaling during Drosophila oogenesis
Molecular biology of the cell, Vol.36(9), 36:ar105
09/01/2025
DOI: 10.1091/mbc.E25-05-0218
PMCID: PMC12415609
PMID: 40632522
Abstract
Growing evidence supports that lipid droplets (LDs) are critical for producing high-quality oocytes. However, the functions of LDs during oocyte development remain largely unknown. Using Drosophila oogenesis as a model, we previously discovered the LD-associated Adipose Triglyceride Lipase (ATGL) promotes actin remodeling necessary for oocyte development by providing the substrate for producing lipid signals termed prostaglandins (PGs). Here we find that Jabba, a LD-associated protein best known for its role in anchoring other proteins to LDs, also promotes PG-dependent actin remodeling. Overexpression of Jabba results in thickened cortical actin and excessive actin bundles, whereas loss of Jabba results in cortical actin breakdown and severely defective actin bundle formation. We find that Jabba regulates actin remodeling in conjunction with PG signaling. However, the lack of a genetic interaction between Jabba and ATGL suggests the Jabba-PG pathway is independent of ATGL. These data are consistent with the model that there are multiple PG signaling pathways promoting actin remodeling. Overexpression of Jabba rescues the actin defects when PG signaling is lost. Together these data lead to the model that PGs produced independently of ATGL positively regulate Jabba to promote actin remodeling necessary for follicle morphogenesis.
Details
- Title: Subtitle
- The lipid droplet protein Jabba promotes actin remodeling downstream of prostaglandin signaling during Drosophila oogenesis
- Creators
- Jonathon M Thomalla - University of RochesterMichelle S Giedt - University of IowaRoger P White - University of RochesterIsrael J Wipf - University of IowaAlicia Shipley - University of RochesterMinhao Chen - University of RochesterMichael A Welte - University of RochesterTina L Tootle - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.36(9), 36:ar105
- DOI
- 10.1091/mbc.E25-05-0218
- PMID
- 40632522
- PMCID
- PMC12415609
- NLM abbreviation
- Mol Biol Cell
- ISSN
- 1939-4586
- eISSN
- 1939-4586
- Publisher
- AMER SOC CELL BIOLOGY
- Grant note
- National Institutes of Health: GM116885, GM144057, GM102155, T32 CA078586, T32 GM144636, HD114777, HD10173, P40OD018537
We thank the Tootle and the Welte laboratories for helpful discussions and careful review of the article and Pakinee Phromsiri with assistance generating pJabba (2) . Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. FlyBase (release FB2024_04) was used for information on stocks. At the University of Iowa, Information Technology Services-Research Services provided data storage support. This project was supported by the National Institutes of Health (GM116885 and GM144057; to T.L.T., GM102155; to M.A.W.) . M.S.G. was partially supported by National Institutes of Health grant T32 CA078586 Free Radical and Radiation Biology, University of Iowa, and I.J.W. was partially supported by National Institutes of Health grant T32 GM144636 Pharmacological Sciences, University of Iowa. J.M.T. was partially supported by National Institutes of Health grants HD114777 and HD10173 to Dr. Mariana Wolfner at Cornell University.
- Language
- English
- Electronic publication date
- 07/09/2025
- Date published
- 09/01/2025
- Academic Unit
- Anatomy and Cell Biology; Biology
- Record Identifier
- 9984845655802771
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