Journal article
The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function
Molecular & Biochemical Parasitology, Vol.132(1), pp.1-16
2003
DOI: 10.1016/S0166-6851(03)00211-1
PMID: 14563532
Abstract
Leishmania sp. are digenetic protozoa that cause an estimated 1.5–2 million new cases of leishmaniasis per year worldwide. Among the molecular factors that contribute to
Leishmania sp. virulence and pathogenesis is the major surface protease, alternately called MSP, GP63, leishmanolysin, EC3.4.24.36, and PSP, which is the most abundant surface protein of leishmania promastigotes. Recent studies using gene knockout, antisense RNA and overexpression mutants have demonstrated a role for MSP in resistance of promastigotes to complement-mediated lysis and either a direct or indirect role in receptor-mediated uptake of leishmania. The
MSP gene clusters in different
Leishmania sp. include multiple distinct
MSPs that tend to fall into three classes, which can be distinguished by their sequences and by their differential expression in parasite life stages. Regulated expression of
MSP class gene products during the parasite life cycle occurs at several levels involving both mRNA and protein metabolism. In this review we summarize advances in MSP research over the past decade, including organization of the gene families, crystal structure of the protein, regulation of mRNA and protein expression, biosynthesis and possible functions. The MSPs exquisitely demonstrate the multiple levels of post-transcriptional gene regulation that occur in
Leishmania sp. and other trypanosomatid protozoa.
Details
- Title: Subtitle
- The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function
- Creators
- Chaoqun Yao - VA Medical Center, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMary E Wilson - VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular & Biochemical Parasitology, Vol.132(1), pp.1-16
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0166-6851(03)00211-1
- PMID
- 14563532
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 2003
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984001121702771
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