Journal article
The 'mitoflash' probe cpYFP does not respond to superoxide
Nature, Vol.514(7523), pp.E12-E14
2014
DOI: 10.1038/nature13858
PMCID: PMC4346172
PMID: 25341790
Abstract
Ageing and lifespan of organisms are determined by complicated interactions between their genetics and the environment, but the cellular mechanisms remain controversial. There have been a number of studies suggesting that cellular energy metabolism and free radical dynamics affect lifespan, implicating mitochondrial function. Recently, Shen et al. provided apparent mechanistic insight by reporting that mitochondrial oscillations of ‘free radical production’, called ‘mitoflashes’, in the pharynx of 3-day old Caenorhabditis elegans correlated inversely with lifespan. The interpretation of ‘mitoflashes’ as ‘bursts of superoxide’ radicals assumes that circularly permuted yellow fluorescent protein (cpYFP) is a reliable indicator of mitochondrial superoxide. This interpretation has been criticised because experiments and theoretical considerations both show that changes in cpYFP fluorescence are due to alterations in pH, not superoxide-. We now provide direct evidence that purified cpYFP is completely unresponsive to superoxide. Therefore ‘mitoflashes’ do not reflect superoxide generation and are not evidence for a link between mitochondrial free radical dynamics and lifespan.
Details
- Title: Subtitle
- The 'mitoflash' probe cpYFP does not respond to superoxide
- Creators
- Markus Schwarzlaender - Institute of Crop Science and Resource ConservationStephan Wagner - Institute of Crop Science and Resource ConservationYulia G Ermakova - Shemyakin Oychinnikov Institute Bioorgan ChemistryVsevolod V Belousov - Shemyakin Oychinnikov Institute Bioorgan ChemistryRafael Radi - Departamento de Bioquı´mica, and Center for Free Radical and BiomedicalResearch, Facultad deMedicinaJoseph S Beckman - Linus Pauling Institute, Environmental Health Sciences Center, Department of Biochemistry and BiophysicsGarry R Buettner - Department of Radiation Oncology and Interdisciplinary Grad Program Human ToxicologyNicolas Demaurex - Department of Cell Physiology and MetabolismMichael R Duchen - Department of Cell and Developmental Biology and Consortium for Mitochondrial ResearchHenry J Forman - Davis School Gerontology, Andrus Gerontoogyl CenterMark D Fricker - Department of Plant SciencesDavid Gems - Dept of Genetics, Evolution and Environment [London]Andrew P Halestrap - School of Biochemistry and Bristol CardioVascularBarry Halliwell - Department of BiochemistryUrsula Jakob - Molecular, Cellular and Development Biology DepartmentIain G Johnstonn - Department of Mathematics [Imperial College London]Nick S Jones - Department of Mathematics [Imperial College London]David LOGAN - Institut de Recherche en Horticulture et SemencesBruce Morgan - Division of Redox Regulation, DKFZ ZMBH AllianceFlorian L Mueller - Department Cancer BiologyDavid G Nicholls - Buck Institute for Research on AgingS. James Remington - Institut of Molecular Biology, Department of PhysicPaul T Schumacker - Division of Neonatology, Department of Pediatrics, Feinberg School of MedicineChristine C Winterbourn - Department Pathology, Centre Free Radical ResearchLee J Sweetlove - Department of Plant ScienceAndreas J Meyer - Institut of Crop Science and Resource Conservation INRESTobias P Dick - Division of Redox Regulation, DKFZ ZMBH AllianceMichael P Murphy - Mitochondrial Biology Unit
- Resource Type
- Journal article
- Publication Details
- Nature, Vol.514(7523), pp.E12-E14
- DOI
- 10.1038/nature13858
- PMID
- 25341790
- PMCID
- PMC4346172
- NLM abbreviation
- Nature
- ISSN
- 0028-0836
- eISSN
- 1476-4679
- Publisher
- Springer Science and Business Media LLC
- Language
- English
- Date published
- 2014
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047788202771
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