The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart

Bahri Karacay; Enzhong Xie; Long-Sheng Chang

doi:10.1016/0378-1119(95)00156-Z

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The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart

Journal article

Peer reviewed

The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart

Bahri Karacay, Enzhong Xie and Long-Sheng Chang

Gene, Vol.158(2), pp.253-256

1995

DOI: 10.1016/0378-1119(95)00156-Z

PMID: 7607550

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Abstract

We have isolated a complete cDNA encoding for the mouse erythrocyte protein 4.2 (P4.2). The entire P4.2 cDNA consists of 3465 nt with an open reading frame (ORF) of 691 amino acids. Northern blot analysis of mouse reticulocyte or spleen RNA using the P4.2 cDNA as a probe, detected a 3.5-kb message. The size of the mouse P4.2 cDNA or message that we obtained, appears to be different from those reported recently. Despite the similarity to the human P4.2 cDNAs, the mouse cDNA has a longer 3′ untranslated region. A genomic clone covering the first exon and flanking sequences of the mouse. P4.2 gene was isolated. Sequencing results from the first exon-intron junction region and polymerase chain reaction (PCR) experiments revealed that the mouse reticulocyte P4.2 RNA does not exhibit alternative splicing in the region identified in the human P4.2 RNA.

transglutaminase

cDNA

genomic sequence

Erythrocyte band 4.2

alternative splicing

Details

Title: Subtitle: The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart
Creators: Bahri Karacay - Division of Hematology and Oncology, Department of Pediatrics, Children's Hospital, The Ohio State University, Columbus, OH 43205, USA
Enzhong Xie - Division of Hematology and Oncology, Department of Pediatrics, Children's Hospital, The Ohio State University, Columbus, OH 43205, USA
Long-Sheng Chang - Division of Hematology and Oncology, Department of Pediatrics, Children's Hospital, The Ohio State University, Columbus, OH 43205, USA
Resource Type: Journal article
Publication Details: Gene, Vol.158(2), pp.253-256
Publisher: Elsevier B.V
DOI: 10.1016/0378-1119(95)00156-Z
PMID: 7607550
ISSN: 0378-1119
eISSN: 1879-0038
Language: English
Date published: 1995
Academic Unit: Stead Family Department of Pediatrics; Iowa Neuroscience Institute
Record Identifier: 9984065831902771

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