The nasal and oropharyngeal microbiomes of healthy livestock workers

Ashley E Kates; Mark Dalman; James C Torner; Tara C Smith

doi:10.1371/journal.pone.0212949

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The nasal and oropharyngeal microbiomes of healthy livestock workers

Journal article

Open access

Peer reviewed

The nasal and oropharyngeal microbiomes of healthy livestock workers

Ashley E Kates, Mark Dalman, James C Torner and Tara C Smith

PloS one, Vol.14(3), pp.e0212949-e0212949

2019

DOI: 10.1371/journal.pone.0212949

PMCID: PMC6413945

PMID: 30861031

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Abstract

Little information exists on the microbiomes of livestock workers. A cross-sectional, epidemiological study was conducted enrolling 59 participants (26 of which had livestock contact) in Iowa. Participants were enrolled in one of four ways: from an existing prospective cohort study (n = 38), from the Iowa Department of Natural Resources Animal Feeding Operations database (n = 17), through Iowa county fairs (n = 3), and through snowball sampling (n = 1). We collected swabs from the nares and oropharynx of each participant to assess the microbiome via 16s rRNA sequencing. We observed livestock workers to have greater diversity in their microbiomes compared to those with no livestock contact. In the nares, there were 27 operational taxonomic units found to be different between livestock workers and non-livestock workers with the greatest difference seen with Streptococcus and Proteobacteria. In the oropharynx, livestock workers with swine exposure were more likely to carry several pathogenic organisms. The results of this study are the first to characterize the livestock worker nasal and oropharyngeal microbiomes.

Carrier State - epidemiology

Prospective Studies

Humans

Middle Aged

Carrier State - microbiology

Male

Healthy Volunteers

Proteobacteria - genetics

Streptococcus - isolation & purification

Aged, 80 and over

Adult

Female

Iowa - epidemiology

Occupational Exposure - statistics & numerical data

Oropharynx - microbiology

DNA, Bacterial - isolation & purification

Cross-Sectional Studies

Occupational Exposure - adverse effects

Nasal Mucosa - microbiology

Animals

Proteobacteria - isolation & purification

Streptococcus - genetics

Microbiota - genetics

Animal Husbandry

RNA, Ribosomal, 16S - genetics

Swine - microbiology

Aged

Details

Title: Subtitle: The nasal and oropharyngeal microbiomes of healthy livestock workers
Creators: Ashley E Kates - Department of Epidemiology, College of Public Health, University of Iowa, Iowa City, IA, United States of America
Mark Dalman - Kent State University, College of Public Health, Kent, OH, United States of America
James C Torner - Department of Epidemiology, College of Public Health, University of Iowa, Iowa City, IA, United States of America
Tara C Smith - Kent State University, College of Public Health, Kent, OH, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.14(3), pp.e0212949-e0212949
DOI: 10.1371/journal.pone.0212949
PMID: 30861031
PMCID: PMC6413945
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: U54 OH007548 / NIOSH CDC HHS
Language: English
Date published: 2019
Academic Unit: Epidemiology; Surgery; Injury Prevention Research Center; Neurosurgery
Record Identifier: 9983995008302771

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