Journal article
The noninvasive mouse ear swelling assay: II. Testing the contact sensitizing potency of fragrances
Fundamental and applied toxicology, Vol.17(4), pp.807-820
1991
DOI: 10.1016/0272-0590(91)90187-9
PMID: 1778366
Abstract
The noninvasive mouse ear swelling assay (MESA) for contact allergy testing was evaluated using fragrance components and complex fragrance mixtures. The test materials represented weak sensitizers and nonsensitizers. Two versions of the MESA were investigated. Both were noninvasive and utilized only topical abdominal dosing and ear challenge with single applications in
BALB
cBy
mice. The vit A MESA differed from the regular MESA only in that mice were maintained on a diet with 17-fold higher levels of vitamin A (vit A) acetate beginning 3 weeks prior to induction. Sensitization reactions were determined by measuring the mean increase in ear swelling over baseline at 24, 48 and 72 hr postexposure. Irritation doseresponse curves facilitated choosing a high nonirritating challenge dose. Sensitization dose-response curves were developed for cinnamaldehyde (CINN) and a complex fragrance mixture, F-16. From these curves, the SD50 was determined. This value represents the dose which sensitized half the animals and serves to rank the potency of compounds for allergic contact dermatitis and to compare values among different assays. The SD50 for CINN was 21.6% while the SD50
vit A for F-16 was 26.6%. The other fragrance, isoeugenol (ISOE), and fragrance mixtures, F-07 and F-22, were also found to be weak sensitizers in the MESA and vit A MESA. The results in the MESA for CINN and ISOE were in the range observed with guinea pig test protocols but showed that the MESA was more sensitive than human test protocols. Two of the fragrance mixtures tested in the MESA gave comparable results in the Buehler guinea pig assay. However, the third (F-22) was negative in the Buehler assay and the MESA, but positive in the vit A MESA. The results of this work with weak sensitizers and the companion study (Thorne
et al., 1991) with potent sensitizers at low doses illustrate that the noninvasive MESA is as sensitive as many standard guinea pig assays. In addition, it is easier and much less expensive to perform. The vit A MESA has the sensitivity and predictive power needed to test compounds and mixtures for contact sensitizing potency.
Details
- Title: Subtitle
- The noninvasive mouse ear swelling assay: II. Testing the contact sensitizing potency of fragrances
- Creators
- Peter S Thorne - Department of Preventive Medicine and Environmental Health, College of Medicine, University of Iowa, Iowa City, Iowa 52242 USACheryl Hawk - Department of Preventive Medicine and Environmental Health, College of Medicine, University of Iowa, Iowa City, Iowa 52242 USASusan D Kaliszewski - Department of Preventive Medicine and Environmental Health, College of Medicine, University of Iowa, Iowa City, Iowa 52242 USAPatrick D Guiney - S. C. Johnson & Son, Inc., Racine, Wisconsin USA
- Resource Type
- Journal article
- Publication Details
- Fundamental and applied toxicology, Vol.17(4), pp.807-820
- DOI
- 10.1016/0272-0590(91)90187-9
- PMID
- 1778366
- NLM abbreviation
- Fundam Appl Toxicol
- ISSN
- 0272-0590
- eISSN
- 1095-6832
- Publisher
- Elsevier Science (USA)
- Language
- English
- Date published
- 1991
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983997305702771
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