Journal article
The nucleolus as a genomic safe harbor for strong in oceanica
Molecular plant, Vol.15(2), pp.340-353
02/07/2022
DOI: 10.1016/j.molp.2021.11.003
PMID: 34775107
Abstract
Microalgae are used in food and feed, and they are considered a potential feedstock for sustainably pro-duced chemicals and biofuel. However, production of microalgal-derived chemicals is not yet economically feasible. Genetic engineering could bridge the gap to industrial application and facilitate the production of novel products from microalgae. Here, we report the discovery of a novel gene expression system in the oleaginous microalga Nannochloropsis that exploits the highly efficient transcriptional activity of RNA po-lymerase I and an internal ribosome entry site for translation. We identified the nucleolus as a genomic safe harbor for Pol I transcription and used it to construct transformant strains with consistently strong trans-gene expression. The new expression system provides an outstanding tool for genetic and metabolic en-gineering of microalgae and thus will probably make substantial contributions to microalgal research.
Details
- Title: Subtitle
- The nucleolus as a genomic safe harbor for strong in oceanica
- Creators
- Christian Sudfeld - Wageningen University & ResearchAna Pozo-Rodriguez - Wageningen University & ResearchSara A. Manjavacas Diez - Wageningen University & ResearchRene H. Wijffels - Nord UniversityMaria J. Barbosa - Wageningen Univ, Bioproc Engn, POB 16, NL-6700 AA Wageningen, NetherlandsSarah D'Adamo - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Molecular plant, Vol.15(2), pp.340-353
- DOI
- 10.1016/j.molp.2021.11.003
- PMID
- 34775107
- NLM abbreviation
- Mol Plant
- ISSN
- 1674-2052
- eISSN
- 1752-9867
- Publisher
- Elsevier
- Number of pages
- 14
- Grant note
- 737.016.007 / Dutch Research Council (NWO) Building Blocks of Life programme
- Language
- English
- Date published
- 02/07/2022
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398030602771
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