The olfactory nerve and not the trigeminal nerve is the major site of CNS entry for mouse hepatitis virus, strain JHM

E M Barnett; S Perlman

doi:10.1006/viro.1993.1248

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The olfactory nerve and not the trigeminal nerve is the major site of CNS entry for mouse hepatitis virus, strain JHM

Journal article

Peer reviewed

The olfactory nerve and not the trigeminal nerve is the major site of CNS entry for mouse hepatitis virus, strain JHM

E M Barnett and S Perlman

Virology, Vol.194(1), pp.185-191

05/1993

DOI: 10.1006/viro.1993.1248

PMID: 8386871

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Abstract

Several viruses, including mouse hepatitis virus strain JHM (MHV-JHM), enter the brain after intranasal inoculation and spread transneuronally to other parts of the central nervous system (CNS). Both the olfactory and trigeminal nerves innervate the nasal cavity and are potential portals of virus entry into the CNS. To evaluate the relative importance of each nerve for MHV infection, mice were infected under conditions that discriminated between trigeminal and olfactory nerve entry. When olfactory nerve entry was selectively eliminated by surgical removal of both olfactory bulbs or by chemical destruction of the olfactory epithelium, MHV-JHM spread into the CNS was completely prevented. On the other hand, direct inoculation into the olfactory bulb, which eliminates all entry via the trigeminal nerve, had no effect on the pattern of virus infection. Thus MHV-JHM enters the CNS via the olfactory nerve after intranasal inoculation while entry via the trigeminal nerve is an insignificant part of this process.

Trigeminal Nerve - microbiology

Olfactory Nerve - microbiology

Specific Pathogen-Free Organisms

Mice, Inbred C57BL

Murine hepatitis virus - growth & development

Animals

Hepatitis, Viral, Animal - transmission

In Situ Hybridization

Olfactory Bulb - surgery

Hepatitis, Viral, Animal - etiology

Mice

Central Nervous System - microbiology

Denervation

Mesencephalon - microbiology

Details

Title: Subtitle: The olfactory nerve and not the trigeminal nerve is the major site of CNS entry for mouse hepatitis virus, strain JHM
Creators: E M Barnett - Neuroscience Program, University of Iowa, Iowa City 52242
S Perlman
Resource Type: Journal article
Publication Details: Virology, Vol.194(1), pp.185-191
DOI: 10.1006/viro.1993.1248
PMID: 8386871
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: MH10384 / NIMH NIH HHS DC01711 / NIDCD NIH HHS NS24401 / NINDS NIH HHS
Language: English
Date published: 05/1993
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777472502771

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