Journal article
The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors
The Journal of cell biology, Vol.183(3), pp.485-498
11/03/2008
DOI: 10.1083/jcb.200806009
PMCID: PMC2575789
PMID: 18981232
Abstract
Photoreceptors are compartmentalized neurons in which all proteins responsible for evoking visual signals are confined to the outer segment. Yet, the mechanisms responsible for establishing and maintaining photoreceptor compartmentalization are poorly understood. Here we investigated the targeting of two related membrane proteins, R9AP and syntaxin 3, one residing within and the other excluded from the outer segment. Surprisingly, we have found that only syntaxin 3 has targeting information encoded in its sequence and its removal redirects this protein to the outer segment. Furthermore, proteins residing in the endoplasmic reticulum and mitochondria were similarly redirected to the outer segment after removing their targeting signals. This reveals a pattern where membrane proteins lacking specific targeting information are delivered to the outer segment, which is likely to reflect the enormous appetite of this organelle for new material necessitated by its constant renewal. This also implies that every protein residing outside the outer segment must have a means to avoid this “default” trafficking flow.
Details
- Title: Subtitle
- The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors
- Creators
- Sheila A Baker - Albert Eye Research Institute, Duke University Medical Center, Durham, NC 27710Mohammad Haeri - Department of Biochemistry and Molecular Biology and Ophthalmology, State University of New York, Upstate Medical University, Syracuse, NY 13210Peter Yoo - Albert Eye Research Institute, Duke University Medical Center, Durham, NC 27710Sidney M Gospe III - Albert Eye Research Institute, Duke University Medical Center, Durham, NC 27710Nikolai P Skiba - Albert Eye Research Institute, Duke University Medical Center, Durham, NC 27710Barry E Knox - Department of Biochemistry and Molecular Biology and Ophthalmology, State University of New York, Upstate Medical University, Syracuse, NY 13210Vadim Y Arshavsky - Albert Eye Research Institute, Duke University Medical Center, Durham, NC 27710
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.183(3), pp.485-498
- DOI
- 10.1083/jcb.200806009
- PMID
- 18981232
- PMCID
- PMC2575789
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 11/03/2008
- Academic Unit
- Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025266802771
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