The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection

K A Brogden; V C Kalfa; M R Ackermann; D E Palmquist; P B McCray Jr; B F Tack

doi:10.1128/AAC.45.1.331-334.2001

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The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection

Journal article

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Peer reviewed

The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection

K A Brogden, V C Kalfa, M R Ackermann, D E Palmquist, P B McCray Jr and B F Tack

Antimicrobial agents and chemotherapy, Vol.45(1), pp.331-334

01/2001

DOI: 10.1128/AAC.45.1.331-334.2001

PMCID: PMC90286

PMID: 11120991

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Abstract

Cathelicidins are antimicrobial peptides from sheep (SMAP29 and SMAP34), rabbits (CAP11 and CAP18), rodents (CRAMP), and humans (FALL39, LL37, and h/CAP18). In a broth microdilution assay against nine ovine pathogens, SMAP29, SMAP34, mouse CRAMP, CAP18, CAP18(31), CAP18(28), CAP18(22), and CAP18(21a) were the most active, with MICs as low as 0.6 microg/ml. Other cathelicidins were less active. In lambs with pneumonia, 0.5 mg of SMAP29 reduced the concentration of bacteria in both bronchoalveolar lavage fluid and consolidated pulmonary tissues. Hence, the antimicrobial activity of SMAP29 suggests that it has applications in the treatment of respiratory tract infections.

Respiratory Tract Infections - microbiology

Lung - microbiology

Animals

Cathelicidins

Sheep Diseases - microbiology

Blood Proteins

Antimicrobial Cationic Peptides - pharmacology

Sheep

Anti-Bacterial Agents - pharmacology

Proteins - pharmacology

Pseudomonas aeruginosa - drug effects

Respiratory Tract Infections - veterinary

Details

Title: Subtitle: The ovine cathelicidin SMAP29 kills ovine respiratory pathogens in vitro and in an ovine model of pulmonary infection
Creators: K A Brogden - Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA. kbrogden@nadc.ars.usda.gov
V C Kalfa
M R Ackermann
D E Palmquist
P B McCray Jr
B F Tack
Resource Type: Journal article
Publication Details: Antimicrobial agents and chemotherapy, Vol.45(1), pp.331-334
DOI: 10.1128/AAC.45.1.331-334.2001
PMID: 11120991
PMCID: PMC90286
NLM abbreviation: Antimicrob Agents Chemother
ISSN: 0066-4804
eISSN: 1098-6596
Language: English
Date published: 01/2001
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Periodontics; Internal Medicine
Record Identifier: 9984093340302771

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