Journal article
The p150 Isoform of ADAR1 Blocks Sustained RLR signaling and Apoptosis during Influenza Virus Infection
PLoS pathogens, Vol.16(9), e1008842
09/08/2020
DOI: 10.1371/journal.ppat.1008842
PMCID: PMC7500621
PMID: 32898178
Abstract
Signaling through retinoic acid inducible gene I (RIG-I) like receptors (RLRs) is tightly regulated, with activation occurring upon sensing of viral nucleic acids, and suppression mediated by negative regulators. Under homeostatic conditions aberrant activation of melanoma differentiation-associated protein-5 (MDA5) is prevented through editing of endogenous dsRNA by RNA editing enzyme Adenosine Deaminase Acting on RNA (ADAR1). In addition, ADAR1 is postulated to play pro-viral and antiviral roles during viral infections that are dependent or independent of RNA editing activity. Here, we investigated the importance of ADAR1 isoforms in modulating influenza A virus (IAV) replication and revealed the opposing roles for ADAR1 isoforms, with the nuclear p110 isoform restricting versus the cytoplasmic p150 isoform promoting IAV replication. Importantly, we demonstrate that p150 is critical for preventing sustained RIG-I signaling, as p150 deficient cells showed increased IFN-β expression and apoptosis during IAV infection, independent of RNA editing activity. Taken together, the p150 isoform of ADAR1 is important for preventing sustained RIG-I induced IFN-β expression and apoptosis during viral infection.
Details
- Title: Subtitle
- The p150 Isoform of ADAR1 Blocks Sustained RLR signaling and Apoptosis during Influenza Virus Infection
- Creators
- Olivia A Vogel - University of Iowa, Microbiology and ImmunologyJulianna Han - University of ChicagoChieh-Yu Liang - University of Iowa, Microbiology and ImmunologySanthakumar ManicassamyJasmine T PerezBalaji Manicassamy - University of Iowa, Microbiology and Immunology
- Resource Type
- Journal article
- Publication Details
- PLoS pathogens, Vol.16(9), e1008842
- DOI
- 10.1371/journal.ppat.1008842
- PMID
- 32898178
- PMCID
- PMC7500621
- NLM abbreviation
- PLoS Pathog
- ISSN
- 1553-7366
- eISSN
- 1553-7374
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: T32GM007183; DOI: 10.13039/100000002, name: National Institutes of Health, award: T32GM007183; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01AI123359- 02S1; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01AI123359; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01AI127775
- Language
- English
- Date published
- 09/08/2020
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984228050602771
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